• Rat BDNF (Brain Derived Neurotrophic Factor) CLIA Kit (STJC0001961)

Rat BDNF (Brain Derived Neurotrophic Factor) CLIA Kit (STJC0001961)

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STJC0001961

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Applications: CLIA
Reactivity: Rat
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 7.5pg/mL
Detection Limit: 12.5~800pg/mL
Short Description: This rat BDNF kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: Bdnf
Gene ID: 24225
Uniprot ID: BDNF_RAT
Specificity: This kit recognizes Rat BDNF in samples. No significant cross-reactivity or interference between Rat BDNF and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications BDNF precursor form: N-glycosylated and glycosulfated, contrary to mature BDNF. Mature BDNF is produced by proteolytic removal of the propeptide, catalyzed by a FURIN family member. In addition, the precursor form is proteolytically cleaved within the propeptide, but this is not an obligatory intermediate for the production of mature BDNF. Can be converted into mature BDNF by plasmin (PLG).
Function Important signaling molecule that activates signaling cascades downstream of NTRK2. During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. BDNF precursor form: Important signaling molecule that activates signaling cascades downstream of NTRK2. Activates signaling cascades via the heterodimeric receptor formed by NGFR and SORCS2. Signaling via NGFR and SORCS2 plays a role in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. Promotes neuronal growth cone collapse.
Protein Name Brain-Derived Neurotrophic Factor
Bdnf Cleaved Into - Bdnf Precursor Form
Probdnf
Database Links Reactome: R-RNO-9026527
Reactome: -RNO-9028731
Reactome: -RNO-9032759
Cellular Localisation Secreted
Bdnf Precursor Form: Secreted
A Proportion Of Bdnf Is Secreted As Immature Precursor (Probdnf)
Alternative CLIA Names Brain-Derived Neurotrophic Factor CLIA kit
Bdnf Cleaved Into - Bdnf Precursor Form CLIA kit
Probdnf CLIA kit
Bdnf CLIA kit
Specificity This kit recognizes Rat BDNF in samples. No significant cross-reactivity or interference between Rat BDNF and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-106 97
EDTA plasma(n=8) 101-114 107
Cell culture media(n=8) 100-117 107

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 39.41 75.46 354.29 42.97 73.19 351.39
Standard deviation 4.27 7.93 31.43 5.46 6.80 38.23
CV (%) 10.83 10.51 8.87 12.71 9.29 10.88
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance