CD Marker Monoclonal Antibodies
Our CD marker monoclonal antibody range is produced by hybridoma fusion of immunised mouse or rabbit B cells with myeloma cells, giving each clone a single, defined epitope and consistent lot‑to‑lot binding. The range spans flow cytometry, western blot, immunohistochemistry and immunofluorescence formats across immunology, cancer and neuroscience targets, and includes antibodies against more than 150 CD markers from CD1a to CD326.
Browse CD Marker AntibodiesTwo Widely Used T Cell and B Cell Marker Clones
OKT3 and 2E2 are raised against two markers that define T cell and B cell lineages in flow cytometry panels. CD3 is a component of the T cell receptor complex and is expressed on all mature T cells. CD19 is a B cell‑specific surface antigen expressed from early pre‑B cell development through to plasma cell differentiation. Both clones below are validated for flow cytometry.
Anti‑CD3 Antibody [B‑B12] (STJ16101101)
B‑B12 is a mouse monoclonal raised against the CD3 epsilon chain, validated for flow cytometry, immunofluorescence and immunohistochemistry. CD3 is a defining marker of mature T lymphocytes and is used in immunophenotyping panels to identify T cell populations.
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Anti‑CD19 Antibody [2E2] (STJ97911)
2E2 is a mouse monoclonal raised against the B‑lymphocyte antigen CD19, validated for flow cytometry, immunofluorescence and ELISA. CD19 is a B cell‑specific surface antigen expressed from early pre‑B cell development through to plasma cell differentiation and is a standard marker for B cell identification in immunophenotyping panels.
View product →CD Marker Antibodies by Target
Each CD marker defines a distinct cell population or biological pathway. Jump straight to the full product listing for a specific target.
CD3 Antibodies
The multi-subunit complex that facilitates T cell receptor signal transduction, present on every mature T lymphocyte.
View CD3 antibodies → Helper T CellCD4 Antibodies
Initiates and enhances the critical early stages of T cell activation, the defining marker for helper T cell profiling.
View CD4 antibodies → B Cell LineageCD19 Antibodies
Assembles with the B cell antigen receptor to lower the activation threshold, tracking B cells from early development to plasma cells.
View CD19 antibodies → Innate ImmunityCD14 Antibodies
Marks monocytes and macrophages and supports innate immune activation pathways, a key reagent for immunophenotyping panels.
View CD14 antibodies → Stem Cell / PrecursorCD117 Antibodies
Receptor tyrosine kinase with a central role in the survival, growth, activation and movement of mast cells and stem cell precursors.
View CD117 antibodies → NK Cell / NeuralCD56 (NCAM1) Antibodies
Neural cell adhesion molecule expressed on neurons, glia and NK cells, involved in cell-cell adhesion and neurite outgrowth.
View CD56 antibodies → Tumour ImmunologyCD47 Antibodies
Studied in monocyte biology and the tumour microenvironment, with a growing role in tumour immune evasion research.
View CD47 antibodies →Key Benefits of CD Marker Antibodies
CD (Cluster of Differentiation) marker antibodies are essential tools for studying the immune system, cancer biology and disease mechanisms. Using CD marker antibodies in your experimental workflows allows you to accurately identify, classify, and monitor specific cell populations within complex biological samples.
Precise cellular identification
Accurately detect immune cell subsets such as T cells, B cells, NK cells, and myeloid cells. Commonly used antibodies like CD14, CD11c and CD33 are critical for immunophenotyping, immune monitoring and functional studies.
Proven performance in multiple applications
Each CD antibody is validated across experimental workflows, including flow cytometry, immunohistochemistry, western blotting and immunoprecipitation. Antibodies like CD14 IHC provide tissue‑level insights, while flow cytometry benefits from markers such as CD123 and CD146.
Monitoring immune responses
Study activation, differentiation, and checkpoint regulation of immune cells with our wide range of antibodies, enabling insights into autoimmunity, infection, and tumour immunology. Popular reagents include anti‑CD19 and CD34, both widely applied in immune monitoring.
Cancer & biomarker research
CD marker antibodies (e.g., CD19, CD20, CD47) are established tumour biomarkers and therapeutic targets, supporting oncology studies and drug development.
Elucidating molecular pathways
CD marker panels allow mapping of cell signalling pathways, immune checkpoints, and ligand‑receptor interactions, supporting basic research and translational studies.
CD Marker Antibodies for Immunology Research
Accurately define immune cell subsets with our range of CD marker antibodies. Our CD3 antibody, alongside CD3e (anti‑CD3e), support T cell receptor signalling research. CD4 antibodies remain essential for helper T cell profiling, while CD8 reagents advance cytotoxic T cell studies. For dendritic cells, CD11c and CD123 are indispensable, while CD19 targets B cell biology. Together, these high‑performance reagents are critical for immunophenotyping, innate immunity, and adaptive immune response studies. Additionally, CD14 supports innate immune activation pathways, CD9 facilitates cell adhesion and checkpoint research, and CD13 enables in‑depth investigations into myeloid differentiation and metabolic pathways.
CD Marker Antibodies for Cancer and Tumour Biology
Explore key CD markers driving cancer research and biomarker discovery. CD33 is central to studies of myeloid‑derived suppressor cells and leukemia. CD47 enables detailed examination of monocyte biology and tumour microenvironments. In hematologic malignancy research, CD34, CD19, and CD56 support precise lineage tracking. Additional targets like CD326 (EpCAM), CD146, and CD47 facilitate investigation into tumour progression, metastasis and immune evasion pathways.
CD Marker Antibodies for Neuroscience Research
CD56 (NCAM) is a neural cell adhesion molecule expressed on neurons, glia and NK cells, involved in cell‑cell adhesion and neurite outgrowth. CD91 (LRP1) is a multifunctional scavenger receptor expressed in the brain that mediates clearance of amyloid‑beta and is involved in Alzheimer's disease pathology. CD248 (Endosialin) is expressed on pericytes and tumour vasculature and has been studied in glioblastoma and other central nervous system tumours.
Species‑Specific CD Marker Antibodies
The molecular structures of CD markers can vary significantly between species; an antibody validated for one species may not bind correctly to its equivalent in another. Selecting a species‑specific CD antibody is a critical step for accurate and reliable results. We offer a wide range of antibodies validated for human, mouse, rat and other species. Our detailed product information clearly identifies the species reactivity of each antibody.
Featured Human CD Markers
| Cell Type | Marker | Function |
|---|---|---|
| T cell | CD3 | Structure composed of multiple subunits that facilitates signal transduction in T‑cells |
| T cell, macrophage/monocyte, granulocyte | CD4 | Initiates or enhances the critical early stages of T‑cell activation |
| T cell | CD8 | Involved in T‑cell mediated killing (cytotoxicity) |
| B cell, dendritic cell, stem cell/precursor | CD19 | Assembles with antigen receptor on B lymphocytes to lower the threshold required for stimulation |
| B cell | CD79A | Necessary for the initiation of B cell signalling pathways when antigen binds to the B‑cell receptor complex |
| Stem cell/precursor | CD117 | Receptor tyrosine kinase plays a crucial role in the survival, growth, activation, and movement of mast cells |
| Stem cell/precursor / endothelial cell | CD34 | Involved in cell adhesion |
| Endothelial cell and several other cells | CD106 | Functions in leucocyte‑endothelial signal transduction |
Featured Mouse CD Markers
| Cell Type | Marker | Function |
|---|---|---|
| T cell | CD3 | Structure composed of multiple subunits that facilitates signal transduction in T‑cells |
| T cell | CD4 | Initiates or enhances the critical early stages of T‑cell activation |
| T cell | CD8 | Involved in T‑cell mediated killing (cytotoxicity) |
| B cell, dendritic cell, stem cell/precursor | CD19 | Assembles with antigen receptor on B lymphocytes to lower the threshold required for stimulation |
| B cell | CD79A | Involved in B cell signal transduction initiation when an antigen binds to the B cell receptor complex |
| T Cell, B cell, dendritic cell, NK cell, stem cell/precursor, macrophage/monocyte, granulocyte | CD117 | Receptor tyrosine kinase involved in signal transduction & differentiation and development in several cell types |
| Stem cell/precursor / endothelial cell | CD34 | Involved in cell adhesion |
| Dendritic cell / macrophage/monocyte | CD106 | Involved in cell adhesion & differentiation and development |
CD Marker Antibodies in Multiple Formats
Choose the format that best suits your experimental needs – from unconjugated antibodies for maximum flexibility to enzyme‑ or fluorophore‑conjugated options for direct or multiplexed detection. All formats are validated for flow cytometry, Western blotting, IHC, and ELISA. Click each row to expand for details.
Unconjugated
Provides versatility for a wide range of protocols. Use with our selection of fluorescent, enzymatic, or biotinylated secondary antibodies – adapt a single antibody for different detection methods.
- Flexible detection across multiple applications
- Reduces need for multiple conjugated antibodies
- Ideal for in‑house labelling or large‑scale screening
Single Fluorophores
FITC, PE, and APC are essential for building multi‑colour panels. They provide distinct signals with minimal spectral overlap, crucial for accurate data analysis.
- FITC – green, excited at 488 nm
- PE – exceptionally bright, excited at 488 nm
- APC – far‑red, minimal overlap with blue‑laser dyes
Tandem Dyes
Two fluorophores covalently bonded (donor + acceptor) to utilise FRET. Our selection includes PE/Cyanine5, PE/Cyanine7 and PerCP‑Cyanine5.5 for deep multiplexing.
- PE/Cyanine5 – red emission from blue laser
- PE/Cyanine7 – far‑red emission from blue laser
- PerCP‑Cyanine5.5 – photostable, no overlap in PE channel
HRP‑Conjugated
Horseradish peroxidase enables direct, enzyme‑driven signal development with DAB, TMB or ECL – no secondary reagents required. Saves time and reduces background.
- Direct detection – no secondary antibody needed
- High sensitivity for low‑abundance CD markers
- Optimised for WB, IHC and ELISA
Biotin‑Conjugated
Ideal for low‑abundance proteins. Use with streptavidin‑HRP (or AP) and substrate for signal amplification via the avidin‑biotin system.
- Two‑step workflow: primary + amplification
- Maximum sensitivity in WB, IHC, and ELISA
- Proven choice for complex tissue samples
Direct Detection & Multiplexing with Conjugated CD Antibodies
Conjugated primary antibodies carry a fluorophore or enzyme label directly on the antibody, enabling one‑step direct detection of an antigen without a secondary antibody incubation and its associated wash steps. Because the label binds the extracellular epitope directly, these formats are particularly important for flow cytometry, where direct labelling is generally preferred for multi‑parameter panels: it requires fewer processing steps and reduces the risk of cross‑reactivity between antibodies raised in the same host species.
- FITC, PE, APC: Standard fluorophores for multicolour flow cytometry and immunofluorescence.
- AbFluor™ and Alexa Fluor: Bright, photostable dyes with narrow emission spectra for deep multi‑target panels.
- Biotin and HRP: Enzyme‑based detection for WB, IHC and ELISA.
Flexible Assay Design with Unconjugated CD Antibodies
Unconjugated formats suit classic two‑step indirect detection and provide high specificity when paired with a validated secondary antibody.
- Workflow flexibility: Use one primary antibody across multiple applications by changing the labelled secondary.
- Enhanced sensitivity: Indirect detection with a labelled secondary provides an amplified signal for low‑expression targets.
- Bulk quantities available: Suited to in‑house conjugation or large‑scale screening at high‑concentration, unconjugated format.
Click a tab above to compare conjugated and unconjugated formats.
Why choose HRP‑ or biotin‑conjugated CD marker antibodies? Both formats provide direct detection (HRP) or amplified signals (biotin‑streptavidin) for low‑abundance markers. They are compatible with Western blotting, IHC and ELISA, giving you reproducible, sensitive results across a range of experimental setups.
Looking for a Specific CD Marker?
Browse the full range of CD marker antibodies, or contact us if a specific target is not listed.
Browse CD Marker AntibodiesSchlossman SF, Boumsell L, Gilks W, et al. CD antigens 1989. In: Knapp W, Dörken B, Gilks WR, et al., eds. Leucocyte Typing IV. Oxford University Press; 1989.
Zola H, Swart B, Banham A, et al. CD molecules 2006 – human cell differentiation molecules. J Immunol Methods. 2007;319(1-2):1-5.
Perfetto SP, Chattopadhyay PK, Roederer M. Seventeen-colour flow cytometry: unravelling the immune system. Nat Rev Immunol. 2004;4(8):648-655.
Maecker HT, McCoy JP, Nussenblatt R. Standardizing immunophenotyping for the Human Immunology Project. Nat Rev Immunol. 2012;12(3):191-200.