Conjugated Primary Antibodies
Directly labelled for secondary-free detection
Conjugated primary antibodies carry a reporter label bound directly to the antibody, removing the need for a secondary antibody step. Our range includes DyLight fluorescent conjugates and biotin conjugated formats, covering fluorescent protein tags, loading controls, and immune and organelle markers.
Browse Conjugated Primary AntibodiesTwo Formats for Secondary-Free Detection
Anti-GFP {DyLight 488} and Anti-CD19 {Biotin} represent the two main conjugation routes in the range: a fluorophore bound directly to the antibody for one-step imaging and flow work, and a biotin label that opens up detection with streptavidin or avidin-conjugated reporters in fluorescent, HRP, alkaline phosphatase or gold-labelled formats.
Anti-GFP Antibody {DyLight 488} (STJ140275)
Detects GFP-tagged constructs directly in the green channel, with no secondary antibody incubation required. The same anti-GFP clone is also available in DyLight 405, 550 and 633, so the detection channel can be matched to the rest of an imaging panel without introducing a secondary reagent.
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Anti-CD19 Antibody [LT19] {Biotin} (STJ16100123)
A biotinylated B cell marker for flow cytometry and immunoprecipitation, detected via streptavidin. Because biotin-streptavidin detection is format flexible, the same biotinylated primary can be paired with fluorescent, HRP, alkaline phosphatase or gold-labelled streptavidin depending on the assay.
View product →DyLight Conjugated Antibodies for Fluorescent Protein Tags
Directly conjugated DyLight antibodies targeting GFP and RFP enable secondary-free detection of fluorescent protein-tagged constructs. Each target is available across four DyLight wavelengths, so the detection channel can be freely chosen to fit the imaging system and other fluorophores in the panel, without needing to match secondary antibody species or risk cross-channel interference from a secondary reagent.
Secondary-free GFP detection, blue channel.
Secondary-free GFP detection, yellow-orange channel.
Secondary-free GFP detection, far-red channel.
Detects mCherry, tdTomato and RFP, blue channel.
Detects mCherry, tdTomato and RFP, green channel.
Conjugated GAPDH Loading Control Antibodies
GAPDH is available as a directly conjugated primary antibody in DyLight, biotin and HRP formats, covering secondary-free western blot normalisation, IF detection and streptavidin-based workflows. A directly conjugated GAPDH antibody removes the secondary incubation step and avoids any risk of the secondary cross-reacting with other antibodies in a multiplex blot, while the DyLight wavelength choice allows the GAPDH channel to be freely assigned within an imaging panel.
Loading control, 17 species, green channel.
Loading control, 17 species, yellow-orange channel.
Loading control, 17 species, far-red channel.
Biotinylated loading control, streptavidin detection.
HRP conjugated loading control, chemiluminescent detection.
DyLight Conjugated Immune Cell and Organelle Marker Antibodies
DyLight-conjugated primary antibodies against immune cell and organelle markers enable direct detection in flow cytometry and immunofluorescence without a secondary antibody step. Directly conjugated CD45 is used as a pan-leukocyte marker in flow panels where same-species primary antibodies would otherwise require separate species-matched secondaries, and Calnexin {DyLight 633} provides secondary-free ER marker detection in multiplexed IF experiments.
Browse Other Conjugation Types
Beyond the DyLight formats above, our conjugated primary antibody range is also available in Alexa Fluor, APC, Biotin, FITC, PE and HRP formats. Select a conjugation type below to browse that part of the range.
Photostable fluorescent conjugates for imaging and flow cytometry.
Browse Alexa Fluor →Allophycocyanin conjugates for multicolour flow cytometry panels.
Browse APC →Biotinylated primaries for streptavidin and avidin-based detection.
Browse Biotin →Green-emitting fluorescent conjugates for IF and flow cytometry.
Browse FITC →Horseradish peroxidase conjugates for colourimetric or chemiluminescent detection in WB and ELISA.
Browse HRP →Phycoerythrin conjugates for high-sensitivity flow cytometry detection.
Browse PE →Learning and Support
Choosing between a conjugated and unconjugated primary antibody?
Conjugated formats remove the secondary antibody step and suit same-species multiplexing, while unconjugated primaries paired with a secondary give signal amplification for low-abundance targets.
Not sure which conjugation format fits your assay? Talk to us and our team can help you select the right product.
Looking for a Specific Target?
Browse the full range of conjugated primary antibodies, or contact us if a specific target or conjugation format is not listed.
Browse Conjugated Primary Antibodies