Phospho Antibodies | St John's Laboratory

Western blot analysis of lysates from HepG2 cells treated with Ca2+ 40uM 30', using NFAT3 (Phospho-Ser676) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-NFATC4-Ser676 antibody (642-691 aa) (STJ90473)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Immunofluorescence analysis of Hep G2 cells using Phospho-NRF2-S40 Rabbit pAb (STJ11105795) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (STJS001166) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Phospho-NFE2L2-S40 antibody (STJ11105795)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

€161.88 - €803.13

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Anti-Phospho-NFE2L2-Ser40 antibody (STJA0009883)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-NFKB1 p105/p50-S337 Rabbit pAb (STJ22308) at 1:1000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 20s.

Anti-Phospho-NFKB1-S337 antibody (STJ22308)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Anti-Phospho-NFKB1-S337 antibody [SRM] (STJ11107615)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Mouse/Rat

€183.13 - €803.13

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Immunohistochemistry of paraffin-embedded human breast carcinoma using Phospho-NFKB1-S893 antibody (STJ22310).

Anti-Phospho-NFKB1-S893 antibody (STJ22310)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC

Reactivity :

Human

€161.88 - €803.13

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Western blot analysis of lysates from HeLa cells, using Phospho-NFKB1-S907 Rabbit pAb (STJ22311). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-NFKB1-S907 antibody (STJ22311)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

€161.88 - €803.13

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Western blot analysis of lysates from HL60 cells using Phospho-NFKB1-S927 Rabbit pAb (STJ22309). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-NFKB1-S927 antibody (STJ22309)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€161.88 - €803.13

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Anti-Phospho-NFKB1-S932 antibody (STJ11101102)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€161.88 - €803.13

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Western blot analysis of lysates from 293 cells, using Phospho-NFKB1-S932 Rabbit pAb (STJ22312). 293 cells treated by TNF-a. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-NFKB1-S932 antibody (STJ22312)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Western blot analysis of various lysates, using Phospho-NF-Kappa B p105-S932 antibody (STJ11105839) at1:2000 dilution. NIH/3T3 cells were treated by TNF-Alpha (20 ng/ml) and Calyculin A (100 nM) at 37 °C for 10 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Phospho-NFKB1-S932 antibody [S5839RM] (STJ11105839)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€183.13 - €803.13

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Western blot analysis of 293 cells using Phospho-NF Kappa B-p105/p50 (S337) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-NFKB1-Ser337 antibody (304-353 aa) (STJ90346)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of various cells using Phospho-NF Kappa B-p105/p50 (S337) Polyclonal Antibody diluted at 1：500

Anti-Phospho-NFKB1-Ser337 antibody (STJ90754)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis of various cells using Phospho-NF Kappa B-p105 (S893) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-NFKB1-Ser893 antibody (860-909 aa) (STJ90343)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-NFKB1-Ser903 antibody (STJA0009818)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis of various cells using Phospho-NF Kappa B-p105 (S907) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-NFKB1-Ser907 antibody (874-923 aa) (STJ90344)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Anti-Phospho-NFKB1-Ser923 antibody (STJA0009819)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis of various cells using Phospho-NF Kappa B-p105 (S927) Polyclonal Antibody

Anti-Phospho-NFKB1-Ser927 antibody (896-945 aa) (STJ91022)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis of various cells using Phospho-NF Kappa B-p105 (S932) Polyclonal Antibody

Anti-Phospho-NFKB1-Ser932 antibody (899-948 aa) (STJ90345)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of lysates from MDA-MB435 cells, using Phospho-NF-Kappa B2-S866 Rabbit pAb (STJ22313) at dilution. MDA-MB435 cells were treated by TNF-Alpha. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-NFKB2-S866 antibody (STJ22313)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

€161.88 - €803.13

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Western blot analysis of NIH/3T3, using Phospho-NF-Kappa B2-S866 antibody (STJ11105847) at 1:500 dilution. NIH/3T3 cells were treated by TNF-Alpha (20 ng/ml) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-Phospho-NFKB2-S866 antibody [S5847RM] (STJ11105847)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

€183.13 - €803.13

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NF-kappaB p100/p52 (Phospho-Ser865) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-NFKB2-Ser865 antibody (833-882 aa) (STJ90341)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IP/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western Blot analysis of 1 MCF-7 treated with LPS, 2 MCF7, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-NFKB2-Ser866/870 antibody (STJ196366)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

€60.00 - €315.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NF-kappaB p100/p52 (Phospho-Ser869) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-NFKB2-Ser869 antibody (836-885 aa) (STJ90342)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of lysates from RAW264.7 cells treated with EGF 200ng/ml 30', using NF-kappaB p100 (Phospho-Ser872) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-NFKB2-Ser872 antibody (838-887 aa) (STJ90535)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis of lysates from HeLa cells, using Phospho-I Kappa B Alpha-S32 Rabbit pAb (STJ116373) at 1:1000 dilution. HeLa cells were treated by TNF-Alpha (20 ng/mL) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-NFKBIA-S32 antibody (STJ116373)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-I Kappa B Alpha-S32 Rabbit mAb (STJ11102564) at 1:1000 dilution. NIH/3T3 and C6 cells were treated by TNF-Alpha (20 ng/mL) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 120s.

Anti-Phospho-NFKBIA-S32 antibody [S4MR] (STJ11102564)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€183.13 - €803.13

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Western blot analysis of lysates from HeLa cells, using Phospho-I Kappa B Alpha-S32/36 Rabbit pAb (STJ11105785) at 1:500 dilution. HeLa cells were treated by TNF-Alpha (20 ng/ml) and Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-NFKBIA-S32/36 antibody (STJ11105785)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

€161.88 - €803.13

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Western blot analysis of lysates from HeLa cells, using Phospho-I Kappa B Alpha-S36 Rabbit pAb (STJ252747). HeLa cells were treated by TNF-Alpha (20 ng/mL) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-NFKBIA-S36 antibody (STJ11101107)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

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Western blot analysis of various lysates using Phospho-I Kappa B Alpha-S36 Rabbit mAb (STJ11102588) at 1:1000 dilution. THP-1 cells were treated by PMA/TPA (80 nM) at 37 °C for overnight or treated by LPS (1 Mu g/mL) at 37 °C for 6 hours. Both NIH/3T3 cells and C6 cells were treated by TNF-Alpha (20 ng/mL) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 3min.

Anti-Phospho-NFKBIA-S36 antibody [S8MR] (STJ11102588)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€183.13 - €803.13

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Anti-Phospho-NFKBIA-Ser-32/Ser-36 antibody [39A1413] (STJA0005442)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

€399.38

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Western blot analysis of lysates from A431 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-NFKBIA-Ser32 antibody (STJ194713)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunofluorescence analysis of rat-spleen tissue. 1, I Kappa B-Alpha (phospho Ser32/S36) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-NFKBIA-Ser32/S36 antibody (15-64 aa) (STJ90311)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€60.00 - €315.00

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Anti-Phospho-NFKBIA-Tyr-305 antibody (STJA0005444)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€456.88

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Anti-Phospho-NFKBIA-Tyr-42 antibody (STJA0005443)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

€456.88

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Immunohistochemistry analysis of paraffin-embedded human lymph node, using IkappaB-alpha (Phospho-Tyr305) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-NFKBIA-Tyr305 antibody (268-317 aa) (STJ90912)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€60.00 - €315.00

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Western blot analysis of lysates from Jarkat cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-NFKBIA-Tyr42 antibody (9-58 aa) (STJ90312)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of lysates from HeLa cellss, using Phospho-I Kappa B Alpha-Y42 Rabbit pAb (STJ22317). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-NFKBIA-Y42 antibody (STJ22317)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

€161.88 - €803.13

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Western blot analysis of various cells using Phospho-I Kappa B-Beta (S23) Polyclonal Antibody

Anti-Phospho-NFKBIB-Ser23 antibody (8-57 aa) (STJ90313)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of 293 cells using Phospho-I Kappa B-Beta (T19) Polyclonal Antibody

Anti-Phospho-NFKBIB-Thr19 antibody (4-53 aa) (STJ90460)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-NFKBIE-Ser22 antibody (131-180 aa) (STJ91018)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Anti-Phospho-NGEF-Tyr-87 antibody (STJA0005409)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

€456.88

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-NIFK-Thr234 antibody (200-249 aa) (STJ91319)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

€60.00 - €315.00

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Western blot analysis of lysates from HCT116 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-NKX2-1-Ser327 antibody (STJ194753)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Anti-Phospho-NLRC4-Ser-533 antibody (STJA0005486)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€459.38

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Anti-Phospho-NLRC4-Ser-533 antibody [M549] (STJA0005487)

Clonality :

Monoclonal

Host :

Rat

Applications :

WB

Reactivity :

Human/Mouse/Rat

€459.38

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Anti-Phospho-NMDA Epsilon 1/2-Tyr1246/1252 antibody (1216-1265 aa) (STJ91307)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of lysates from HeLa cells, using Phospho-NMDAR1-S896/S897 Rabbit pAb (STJ11103722) at 1:1000 dilution. HeLa cells were treated by UV at room temperature for 30 minutes and PMA (200 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-NMDAR1-S896/S897 antibody (STJ11103722)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €803.13

Choose Options