Methyl Antibodies | St John's Laboratory

Western blot analysis of various lysates, using TriMethyl-Histone H3-K36 rabbit polyclonal antibody (STJ23992) at 1:400 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-Tri-Methyl-Histone H3-K36 antibody (32-42) (STJ23992)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/IHC-P/IF/ICC/ELISA/ChIP/ChIP-seq

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and H3K36me3 rabbit monoclonal antibody (STJ11103285). The ChIP sequencing results indicate the enrichment pattern of H3K36me3 in selected genomic region and representative gene loci (GAPDH) , as shown in figure.

Anti-Tri-Methyl-Histone H3-K36 antibody [S5MR] (STJ11103285)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/DB/IHC-P/IP/ELISA/ChIP/ChIP-seq

Reactivity :

Human/Mouse/Rat/Other

£146.50 - £642.50

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Chromatin immunoprecipitation analysis of extracts of 293F cells, using TriMethyl-Histone H3-K4 Rabbit polyclonal antibody antibody (STJ23983) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-Tri-Methyl-Histone H3-K4 antibody (2-19) (STJ23983)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/IHC-P/ELISA/ChIP/ChIP-seq

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Western blot analysis of hela cells using primary antibody diluted at 1:1000 (4°C overnight). Secondary antibody：Goat Anti-rabbit IgG IRDye 800 ( diluted at 1:5000, 25°C, 1 hour)

Anti-Tri-Methyl-Histone H3-K5 antibody (STJ98848)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of HeLa cells using TriMethyl-Histone H3-K56 polyclonal antibody (STJ29403) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Tri-Methyl-Histone H3-K56 antibody (STJ29403)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA/ChIP-seq

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Immunofluorescence analysis of NIH/3T3 cells using TriMethyl-Histone H3-K64 Rabbit polyclonal antibody (STJ11100934) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Tri-Methyl-Histone H3-K64 antibody (STJ11100934)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Anti-Tri-Methyl-Histone H3-K79 antibody (STJ23995)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/IHC-P/ELISA/ChIP

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K9 antibody (STJ23986) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Tri-Methyl-Histone H3-K9 antibody (STJ23986)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/IHC-P/IF/ICC/ELISA/ChIP

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Anti-Tri-Methyl-Histone H3-K9 antibody [SAA1473] (STJA0019839)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA

Reactivity :

Human

£341.50 - £1,447.50

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Western blot analysis of 1) Hela, 2) Rat Testis Tissue, diluted at 1:1000. Secondary antibody (STJ99666) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Tri-Methyl-Histone H3-Lys14 antibody (STJ197290)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat

£48.00 - £252.00

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Western blot analysis of 1) Hela, 2) 3T3, diluted at 1:1000. Secondary antibody (STJ99666) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Tri-Methyl-Histone H3-Lys18 antibody (STJ197289)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Xiao, Zhen, et al. "Targeting P16INK4A in uterine serous carcinoma through inhibition of histone demethylation. " Oncology reports 41. 5 (2019) : 2667-2678.

Anti-Tri-Methyl-Histone H3-Lys27 antibody (STJ197288)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 1) Hela, 2) 3T3, 3) Rat Liver Tissue, diluted at 1:1000. Secondary antibody (STJ99666) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Tri-Methyl-Histone H3-Lys36 antibody (STJ197287)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of Hela, diluted at 1) 1:1000 , 2) 1:5000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Tri-Methyl-Histone H3-Lys36 antibody [Q12] (STJ96990)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 1) Hela, 2) 3T3, 3) Raw264. 7, diluted at 1:2000. Secondary antibody (STJ99666) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Tri-Methyl-Histone H3-Lys4 antibody (STJ197286)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Tri-Methyl-Histone H3-Lys79 antibody (STJ197285)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of Hela, diluted at 1:500. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Tri-Methyl-Histone H3-Lys79 antibody [1B7] (STJ96994)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of lysates from 1) Hela, 2) Rat Testis tissue, 3) Raw264.7 cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, Dylight 800 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour. (Red) Actin Beta Polyclonal Antibody (STJ91464) antibody was diluted at 1:5000 as loading control, 4°C over night, Dylight 680 secondary antibody (NA) was diluted at 1:10000, 37°C 1hour.

Anti-Tri-Methyl-Histone H3-Lys79 antibody [2C3] (STJ97002)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of Hela cell. 1, Bek Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Tri-Methyl-Histone H3-Lys79 antibody [3G3] (STJ96993)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of 1) Hela, 2) Rat Testis tissue, 3) Raw264.7, diluted at 1:1000. cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Tri-Methyl-Histone H3-Lys9 antibody [2E11] (STJ97006)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of Hela, diluted at 1) 1:2, 000, 2) 1:5, 000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Tri-Methyl-Histone H3-Lys9 antibody [4H8] (STJ97011)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Immunofluorescence analysis of 293T cells using TriMethyl-Histone H4-K20 Rabbit polyclonal antibody (STJ23972). Blue: DAPI for nuclear staining.

Anti-Tri-Methyl-Histone H4-K20 antibody (STJ23972)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Western blot analysis of 1) Hela, 2) 3T3, diluted at 1:2000. Secondary antibody (STJ99666) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Tri-Methyl-Histone H4-Lys20 antibody (STJ197281)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Tri-Methyl-Histone H4-Lys59 antibody (STJ197280)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Tri-Methyl-Histone H4-Lys79 antibody (STJ197279)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-Tri-Methyl-TP53-Lys370 antibody (STJA0005917)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Anti-TriMethyl-AKT-K64 antibody (STJ11107131)

Clonality :

Polyclonal

Host :

Rabbit

Reactivity :

Human

£129.50 - £642.50

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Anti-TriMethyl-Histone H1.4-K26 antibody (STJ11106421)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

£129.50 - £642.50

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Anti-TriMethyl-Histone H3-K27 antibody [SRM] (STJ11107029)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £574.50

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Anti-TriMethyl-Histone H3-K27 antibody [SRM] (STJ11107030)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/DB/IHC-P/IF/ICC/ELISA/ChIP

Reactivity :

Human/Mouse/Rat/Other

£146.50 - £642.50

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Anti-TriMethyl-Histone H3-K27 antibody [SRM] (STJ11107031)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £574.50

Choose Options