Monoclonal Antibody | Primary Antibodies | St John's Laboratory

Western blot analysis of various lysates using Phospho-TBK1/NAK-S172 Rabbit mAb (STJ11102607) at 1:1000 dilution. Both HeLa cells and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1min.

Anti-Phospho-TBK1-S172 antibody [S7MR] (STJ11102607)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

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Western blot analysis of lysates from NIH 3T3 or NIH 3T3 treat with Calyculin A, using Pan Phospho-Threonine Rabbit monoclonal antibody (STJ11105878) at 1:1000 dilution. NIH/3T3 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-Phospho-Threonine antibody [S5878RM] (STJ11105878)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

£146.50 - £642.50

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Immunohistochemistry analysis of paraffin-embedded Human heart using Phospho-Cardiac Troponin I (TNNI3) -S23/S24 Rabbit monoclonal antibody (STJ11105867) at dilution of 1:700 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-TNNI3-S23/S24 antibody [S5867RM] (STJ11105867)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human

£146.50 - £642.50

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Western blot analysis of lysates from HT-29 cells, using Phospho-p53-S33 Rabbit mAb (STJ11102575) at 1:1000 dilution. HT-29 cells were treated by nocodazole (100 ng/mL) at 37 °C for 16 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3min.

Anti-Phospho-TP53-S33 antibody [S5MR] (STJ11102575)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Western blot analysis of lysates from 293T cells, using Phospho-p53-S376 Rabbit mAb (STJ11102578) at 1:1000 dilution. 293T cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-TP53-S376 antibody [S8MR] (STJ11102578)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£146.50 - £642.50

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Western blot analysis of lysates from C6 cells, using Phospho-p53-S392 (STJ11102634) at 1:1000 dilution. C6 cells were treated by Paclitaxel (100 nM) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-Phospho-TP53-S392 antibody [S4MR] (STJ11102634)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

£146.50 - £642.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-p53-S9 Rabbit mAb (STJ11102576) at 1:1000 dilution. NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 3min.

Anti-Phospho-TP53-S9 antibody [S6MR] (STJ11102576)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£146.50 - £642.50

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Western blot analysis of lysates from 293T cells, using Phospho-p53-T55 Rabbit mAb (STJ11102577) at 1:1000 dilution. 293T cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour or treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Phospho-TP53-T55 antibody [S7MR] (STJ11102577)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Western blot analysis of lysates from HeLa cells, using Phospho-Trim24-S1043 Rabbit mAb (STJ11105806) at1:1000 dilution. Hela cells were treated by TGF-Beta (10 ng/ml) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Phospho-Trim24-S1043 antibody [S5806RM] (STJ11105806)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Western blot analysis of NIH/3T3, using Phospho-KAP1/TRIM28-S824 antibody (STJ11105864) at 1:5000 dilution. NIH/3T3 cells were treated by Etoposide (25 uM) at 37 °C for 5 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-Phospho-TRIM28-S824 antibody [S5864RM] (STJ11105864)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

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Anti-Phospho-Tryosine antibody [P-TYR-01] (STJ16100174)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/FC

Reactivity :

Species Independent

£485.50

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Anti-Phospho-Tryosine antibody [P9V6] (STJ16100173)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

£505.50

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Western blot analysis of 1) Jurkat, 2) 293T, 3) Rat Brain Tissue, 4) Mouse Brain Tissue with Phosphotyrosine Mouse mAb diluted at 1:2, 000

Anti-Phospho-Tyrosine antibody [2C1] (STJ97487)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Species independent

£48.00 - £252.00

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Western blot analysis of extracts from A549 cells untreated and treated with Epidermal Growth Factor (10ng/ml, 15min) , using Phospho-Tyrosine Mouse mAb.The phospho-specificity of the antibody was further characterized by treating the membrane with alkaline phosphatase after Western transfer.

Anti-Phospho-Tyrosine antibody [7B4-D9-H10] (STJ99257)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£144.00 - £348.00

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Anti-Phospho-VANGL2-S5/S8 antibody [S5796RM] (STJ11105796)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Western blot analysis of various lysates using Phospho-VANGL2-S79/S82/S84 Rabbit mAb (STJ11103744) at1:1000 dilution. HeLa and C6 cells were treated by wnt (100ng/ml) for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-VANGL2-S79/S82/S84 antibody [S4MR] (STJ11103744)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£146.50 - £642.50

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Anti-Phospho-VANGL2-T78/S79 antibody [S2MR] (STJ11103742)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Western blot analysis of various lysates using Phospho-VANGL2-T78/S79/S82 Rabbit mAb (STJ11103743) at 1:1000 dilution. HeLa cells and NIH/3T3 cells and C6 cells were treated by wnt (100ng/ml) for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-VANGL2-T78/S79/S82 antibody [S3MR] (STJ11103743)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-VASP-S157 Rabbit monoclonal antibody (STJ11105906) at 1:4000 dilution. NIH/3T3 cells were treated by Forskolin (30 uM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Phospho-VASP-S157 antibody [S5906RM] (STJ11105906)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£146.50 - £642.50

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Western blot analysis of lysates from HEL cells, using Phospho-VASP-S157 Rabbit monoclonal antibody (STJ11105907) at 1:3000 dilution. HEL cells were treated by Forskolin (30 uM) at 37 °C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Phospho-VASP-S157 antibody [S5907RM] (STJ11105907)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£146.50 - £642.50

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Western blot analysis of lysates from A-431 cells using Phospho-VEGF Receptor 2-Y1175 Rabbit mAb (STJ11107732) at 1:3900 dilution incubated overnight at 4C. A-431 cells were treated by EGF (50 ng/ml) at 37C for 30 minutes after serum-starvation overnight. <br> Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 30 Mu g per lane. <br> Blocking buffer: 3 % nonfat dry milk in TBST. <br> Detection: ECL Basic Kit <br> Exposure time: 1s.

Anti-Phospho-VEGF Receptor 2-Y1175 antibody [SRM] (STJ11107732)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

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Immunofluorescence analysis of Jurkat treated with H2O2 and Jurkat using Phospho-ZAP70-Y493 Rabbit monoclonal antibody (STJ11105888) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-Phospho-ZAP70-Y493 antibody [S5888RM] (STJ11105888)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IF/ICC/ELISA

Reactivity :

Human

£146.50 - £642.50

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Western blot analysis of extracts of various cell lines, using PHOX2B Rabbit monoclonal antibody (STJ11102326) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-PHOX2B antibody (215-314) [S6MR] (STJ11102326)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Formalin-fixed, paraffin-embedded human neuroblastoma stained with anti-PHOX2B antibody using peroxidase-conjugate and DAB chromogen. Note nuclear staining of tumor cells

Anti-PHOX2B antibody [ZR292] (STJ180589)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC-P

Reactivity :

Human

£181.50 - £703.50

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Immunohistochemistry analysis of paraffin-embedded rat spleen using PI3 Kinase p110 delta Rabbit monoclonal antibody (STJ11103107) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-PI3 Kinase p110 delta antibody (200-300) [S7MR] (STJ11103107)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of various lysates using PI3 Kinase p85 alpha Mouse mAb (STJ11106530) at 1:1000 dilution. <br/> Secondary antibody:HRP Goat Anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br/> Lysates/ proteins: 25 Mu g per lane. <br/> Blocking buffer: 3 % nonfat dry milk in TBST. <br/> Detection: ECL Basic Kit <br/> Exposure time: 30s.

Anti-PI3 Kinase p85 alpha antibody [SMM] (STJ11106530)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various lysates, using PI3 Kinase p85 alpha/beta Rabbit monoclonal antibody (STJ11104858) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-PI3 Kinase p85 alpha/beta antibody (400-500) [S4858RM] (STJ11104858)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Anti-PI3 Kinase p85 alpha/beta antibody [SRM] (STJ11106938)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Confocal imaging of NIH/3T3 cells using PI3KP85 Alpha/P55 Gamma/P85 Beta Rabbit monoclonal antibody (STJ11104970, dilution 1:200) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (Blue). Objective: 60x.

Anti-PI3 Kinase p85 alpha/beta/gamma mAb antibody (400-500) [S4970RM] (STJ11104970)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Mouse/Rat

£146.50 - £642.50

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Western blot analysis of extracts of Mouse lung, using PIAS1 Rabbit monoclonal antibody (STJ11102146) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.

Anti-PIAS1 antibody (3-101) [S6MR] (STJ11102146)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Western blot analysis of various lysates using PIAS1+PIAS2 Rabbit mAb (STJ11102524) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-PIAS1/PIAS2 antibody (552-651) [S4MR] (STJ11102524)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of extracts of various cell lines, using PIAS2 Rabbit monoclonal antibody (STJ11102291) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-PIAS2 antibody (50-150) [S1MR] (STJ11102291)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£129.50 - £574.50

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Immunoprecipitation analysis of 600 Mu g extracts of Rat brain cells using 3 Mu g PIK3C3/VPS34 antibody (STJ11101401). Western blot was performed from the immunoprecipitate using PIK3C3/VPS34 antibody (STJ11101401) at a dilution of 1:500.

Anti-PIK3C3 antibody (788-887) [S1MR] (STJ11101401)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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K562 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-PIK3CA H1047R (ABT453) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: K562Predicted band size: 124kDaObserved band size: 124kDa

Anti-PIK3CA antibody (1000-1068 aa) [ABT453] (STJ197258)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human

£174.00 - £252.00

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Western blot analysis of various lysates using PI3 Kinase p110 beta Rabbit monoclonal antibody (STJ11103834) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PIK3CB antibody (1-100) [S3834RM] (STJ11103834)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Anti-PIK3CB antibody (410-600) [S4849RM] (STJ11104849)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of PI3 Kinase p110 gamma in K562 lysates using PI3 Kinase p110 gamma antibody.

Anti-PIK3CG antibody (3-17 aa) [R09-5C8] (STJA0011789)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human

£264.50

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Western blot analysis of various lysates, using PI3 Kinase p85 alpha rabbit monoclonal antibody (STJ11102215) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PIK3R1 antibody (1-100) [S5MR] (STJ11102215)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunocytochemistry analysis of PI3 Kinase p85 alpha (green) in MCF-7 using PI3 Kinase p85 alpha antibody, and DAPI (blue)

Anti-PIK3R1 antibody (601-619 aa) [R03-3C7] (STJA0013015)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Rat/Hamster

£264.50

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Anti-PIK3R1 antibody (C-Term) [M253] (STJA0005530)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

£394.50

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Western blot analysis of 1) 3T3, 2) Rat LiverTissue with PI3 Kinase P85 Alpha Mouse mAb diluted at 1:2, 000.

Anti-PIK3R1 antibody [3B7] (STJ97521)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Rat/Mouse

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using PI3 Kinase p85 beta antibody (STJ11102171) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PIK3R2 antibody (100-200) [S1MR] (STJ11102171)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

£129.50 - £574.50

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Immunohistochemistry analysis of paraffin-embedded Human colon cancer using PI 3 Kinase p85 beta antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-PIK3R2 antibody (140-164 aa) [R06-5H3] (STJA0014501)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP/FC

Reactivity :

Human/Rat

£264.50

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Western blot detection of PI3 Kinase p85 beta in C6, K562, MCF7, U87MG, HCT116, 3T3 and Hela cell lysates using PI3 Kinase p85 beta mouse mAb (1:1000 diluted).Predicted band size:85KDa.Observed band size:85KDa.

Anti-PIK3R2 antibody [8D9-D5-F8] (STJ99192)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£144.00 - £348.00

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Western blot analysis of extracts of various cell lines, using PIK3R3/p55PIK Rabbit monoclonal antibody (STJ11101979) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PIK3R3 antibody (362-461) [S9MR] (STJ11101979)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Confocal imaging of NIH/3T3 cells using PIK3R4/VPS15 Rabbit monoclonal antibody (STJ11103589, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-PIK3R4/VPS15 antibody (1259-1358) [S9MR] (STJ11103589)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of extracts of 22Rv1 cells, using PIM1 antibody (STJ11101771) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PIM1 antibody (1-100) [S1MR] (STJ11101771)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various lysates, using PIM2 Rabbit mAb (STJ11102458) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-PIM2 antibody (10-120) [S8MR] (STJ11102458)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Monoclonal Antibodies