Primary Antibodies

Monoclonal Antibodies

Traceable to a single parent hybridoma clone

Monoclonal antibodies are produced in laboratories to enhance, restore or mimic the immune system's attack on cancer cells. They are made by cloning a specific white blood cell, so every monoclonal antibody can be traced back to a single parent cell.

How it works

Hybridoma-Based Production

When an antigen is introduced to either a mouse or a rabbit and polyclonal B cells are fused from the spleen to the myeloma, a hybridoma is created. That hybridoma is then cultured to continue antibody production.

Category benefits

Why use monoclonal antibodies?

✓ A single, consistent epitope
Because every monoclonal antibody descends from one parent cell, it binds the same epitope every time, giving consistent results across repeat experiments.
✓ Monovalent affinity by design
Monoclonal antibodies can also be engineered to have monovalent affinity for their target.
✓ A wide range of host species
Our range includes 39,843 monoclonal antibodies across a range of host species, including mouse, rabbit, and cell-line-derived hosts such as 293F and CHO cells, alongside Armenian hamster, chicken, canine and alpaca hosts.
Choose by antibody type

Monoclonal Antibody Formats

Monoclonal antibodies in our range are supplied across several production formats and host species. Mouse and rabbit clones cover the majority of the catalogue, recombinant expression underpins our rabbit monoclonal line, and conjugated formats are available for direct detection assays such as flow cytometry. Choose a format below to browse that part of the range.

Hybridoma Mouse Monoclonal Antibodies Produced by hybridoma fusion of immunised mouse B cells with myeloma cells, giving each clone a single, defined epitope.
Browse Mouse Monoclonals →
Recombinant Rabbit Monoclonal Antibodies Combines rabbit epitope recognition with recombinant expression of defined heavy and light chain sequences, giving batch to batch reproducibility.
Browse Rabbit Monoclonals →
Technology Recombinant Monoclonal Antibodies Generated by cloning antibody genes into an expression vector rather than through hybridoma fusion. Every recombinant antibody in our range is monoclonal, expressed from a defined heavy and light chain sequence.
Browse Recombinant Range →
Direct Detection Conjugated Primary Monoclonal Antibodies Carries a label directly on the antibody for one-step detection of extracellular epitopes. Preferred for multi-parameter flow cytometry: fewer steps, lower cross-reactivity risk.
See Conjugation Types →
Where they're used

Typical applications

✓ Western blotting
Our monoclonal antibodies detect denatured target proteins by western blot, with single-epitope binding supporting reproducible band detection across repeat runs.
✓ Immunohistochemistry and immunocytochemistry
Monoclonal antibodies localise target antigens in tissue sections and fixed cells, supporting expression and localisation studies.
✓ Flow cytometry
Monoclonal antibodies are used to identify and quantify surface or intracellular markers across cell populations.
✓ Immunoprecipitation
Monoclonal antibodies pull down target antigens from complex lysates, which can aid detection of low-abundance proteins.

Need help choosing an antibody?

Contact our technical support team for help selecting an antibody for your target of interest. Each product page includes a downloadable datasheet and SDS, with dispatch on overnight priority shipping with gel ice packs.

Looking for more primary antibodies?

Browse our full range of recombinant, monoclonal and polyclonal primary antibodies.

 View all primary antibodies

For Research Use Only. Not for use in diagnostic procedures.

912 of 2206 Items
0 selected
!
Well-cited Academic Publications
KO-Validated 700+ Lines
Technical Support Expert Scientific Team
1-Year Guarantee Antibodies & Proteins