Loading Control Antibody | St John's Laboratory

Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g Hsp90 Alpha antibody (STJ11102219). Western blot was performed from the immunoprecipitate using Hsp90 Alpha antibody (STJ11102219) at a dilution of 1:1000.

Anti-HSP90AA1 antibody (1-100) [S9MR] (STJ11102219)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Western blot analysis of Jurkat cells using HSP 90 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-HSP90AA1 antibody (251-300 aa) (STJ96692)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of various lysates, using HSP90AA1 Rabbit polyclonal antibody (STJ11105239) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-HSP90AA1 antibody (433-732 aa) (STJ11105239)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Immunofluorescence analysis of U2OS cells using Hsp90 Alpha Rabbit polyclonal antibody (STJ24087). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution.

Anti-HSP90AA1 antibody (433-732) (STJ24087)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using Hsp90 Alpha/ Beta Rabbit monoclonal antibody (STJ11102227) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-HSP90AA1 antibody (533-732) [S7MR] (STJ11102227)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of various lysates using Hsp90 Alpha Rabbit pAb (STJ114322) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-HSP90AA1 antibody (629-732) (STJ114322)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of 1) Hela, 2) Mouse Brain Tissue, 3) Rat Brain Tissue with HSP90 a Mouse mAb diluted at 1:2, 000.

Anti-HSP90AA1 antibody (660-740 aa) [1F6] (STJ97496)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of Hela cell. 1, HSP90A Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Active Caspase-3 monoclonal antibody (5E1) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-HSP90AA1 antibody (683-732 aa) (STJ93622)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot detection of Hsp90 in Hela, 3T3, C6, K562 and A549 cell lysates using Hsp90 mouse mAb (1:2000 diluted).Exposion time: 4min.Predicted band size:90KDa.Observed band size:90KDa.

Anti-HSP90AA1 antibody [4B6-G2-H6] (STJ99220)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat

£144.00 - £348.00

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Western blot detection of Hsp90 alpha in R Brain, Jurkat, CHO-K1, COS7, C6, 3T3 and Hela cell lysates using Hsp90 alpha mouse mAb (1:1000 diluted). Predicted band size:90KDa. Observed band size:90KDa.

Anti-HSP90AA1 antibody [6H7-A3-2G6] (STJ99288)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Rat/Monkey

£144.00 - £348.00

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Immunofluorescence analysis of U2OS cells using HSP70 Rabbit polyclonal antibody (STJ114814) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-HSPA1A antibody (500-600) (STJ114814)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using HSP70/HSPA1 antibody (STJ24092) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-HSPA1A antibody [S2MM] (STJ24092)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£129.50 - £574.50

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Western blot of HeLa cell lysate showing specific immunolabeling of the ~60 kDa HSP60 protein.

Anti-HSPD1 antibody (Full Length) [1C7] (STJA0003845)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC

Reactivity :

Human/Mouse/Rat/Bovine/Canine/Feline/Goat/Guinea Pig/Hamster/Horse/Non-Human Primates/Rabbit/Sheep/Zebrafish

£454.50

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Anti-HSPD1 antibody (N-Term) [M438] (STJA0005440)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat

£390.50

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Western blot analysis of lysates from NIH/3T3 cells, using Lactic acid-Histone H3-K18 Rabbit monoclonal antibody (STJ11104234) at1:2000 dilution. NIH/3T3 cells were treated by Sodium lactate (100mM) for 24h. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-Lactic acid-Histone H3-K18-K18 antibody [S4234RM] (STJ11104234)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Other

£146.50 - £642.50

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STJ72853 (1µg/ml) staining of HeLa (A) and Jurkat (B) nuclear lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Lamin B1 antibody (526-537) (STJ72853)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Dog/Pig/Cow

£312.50

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Anti-Lamin B1 antibody [SRM13030] (STJ11107760)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human/Mouse/Rat

£0.00

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STJ72852 (0. 2µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Lamin B1-Isoform 1 antibody (Internal) (STJ72852)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Pig/Cow

£312.50

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Anti-Lamin-B1 antibody [SRM15107] (STJ11109837)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Western blot analysis of MCF7, SW480, PC-3, SKOV3, mouse spleen, mouse brain, mouse liver, mouse thymus cells using Lamin B1 Polyclonal Antibody. Antibody was diluted at 1:500. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-LMNB1 antibody (391-440 aa) (STJ96545)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of extracts of various cell lines, using Lamin B1 antibody (STJ24413) at 1:1000 dilution. Jurkat cells were treated by Etoposide (25 uM) at 37 °C for 5 hours. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-LMNB1 antibody (397-588) (STJ24413)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA/ChIP

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using [KO Validated] Lamin B1 Rabbit pAb (STJ119228) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-LMNB1 antibody (503-588) (STJ119228)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £642.50

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Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Lamin B1 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-LMNB1 antibody (527-540 aa) [R05-8D6] (STJA0010488)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse/Rat

£679.50

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Western blot detection of Lamin B1 in COS7, Molt-4, A549 and 3T3 cell lysates using Lamin B1 mouse mAb (dilution 1:500).Predicted band size:68kDa.Observed band size:68kDa.

Anti-LMNB1 antibody [5G8-D3-H7] (STJ99312)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse/Monkey

£144.00 - £348.00

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Immunofluorescence analysis of Hela cell. 1, AMPK Alpha 1/2 (phospho Thr183/172) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-LMNB1 antibody [7C11] (STJ96938)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-LMNB1 antibody [7C11] {AbFluor™ 350} (STJA0006109)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Rat/Mouse

£144.00 - £240.00

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Anti-LMNB1 antibody [7C11] {AbFluor™ 405} (STJA0006110)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Rat/Mouse

£144.00 - £240.00

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Anti-LMNB1 antibody [7C11] {AbFluor™ 488} (STJA0006111)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Rat/Mouse

£144.00 - £240.00

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Anti-LMNB1 antibody [7C11] {AbFluor™ 555} (STJA0006112)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Rat/Mouse

£144.00 - £240.00

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Anti-LMNB1 antibody [7C11] {AbFluor™ 594} (STJA0006113)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Rat/Mouse

£144.00 - £240.00

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Anti-LMNB1 antibody [7C11] {AbFluor™ 647} (STJA0006114)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Rat/Mouse

£144.00 - £240.00

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Anti-LMNB1 antibody [7C11] {AbFluor™ 680} (STJA0006115)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Rat/Mouse

£144.00 - £240.00

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Anti-LMNB1 antibody [7C11] {Biotin} (STJA0006116)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Rat/Mouse

£144.00 - £240.00

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Anti-LMNB1 antibody [7C11] {Cy3} (STJA0006117)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Rat/Mouse

£144.00 - £240.00

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Anti-LMNB1 antibody [7C11] {Cy5} (STJA0006118)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Rat/Mouse

£144.00 - £240.00

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Anti-LMNB1 antibody [7C11] {FITC} (STJA0006119)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Rat/Mouse

£144.00 - £240.00

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Anti-LMNB1 antibody [7C11] {HRP} (STJA0006120)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Rat/Mouse

£144.00 - £240.00

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Western blot analysis of extracts of various cell lines, using MonoMethyl-Histone H3-K27 antibody (STJ23987) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.

Anti-Mono-Methyl-H3K27me1-K27 antibody (STJ23987)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/IHC-P/IF/ICC/ELISA/ChIP

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Western blot analysis of 1) Hela, 2) Rat Testis Tissue, diluted at 1:1000.. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Mono-Methyl-Histone H3-Arg17 antibody (STJ97206)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Rat

£48.00 - £252.00

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Western blot analysis of 1) Raw264.7, diluted at 1:1000.. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Mono-Methyl-Histone H3-Arg2 antibody (STJ97203)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Mouse

£48.00 - £252.00

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Western blot analysis of 1) Hela, 2) 3T3, diluted at 1:1000.. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Mono-Methyl-Histone H3-Arg26 antibody (STJ97210)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Mouse

£48.00 - £252.00

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Immunofluorescence analysis of U-2 OS cells using MonoMethyl-Histone H3-K14 polyclonal antibody (STJ27230) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-Mono-Methyl-Histone H3-K14 antibody (STJ27230)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina (RK20265) from 10⁵ K562 cells with 1 Mu g MonoMethyl-Histone H3-K18 rabbit monoclonal antibody (STJ11103411) , along with a Goat Anti-rabbit IgG (H+L). The CUT&Tag results indicate the enrichment pattern of H3K18Me1 in representative gene loci (MYT1) , as shown in figure.

Anti-Mono-Methyl-Histone H3-K18 antibody [S1MR] (STJ11103411)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/DB/IHC-P/IF/ICC/IP/ELISA/ChIP/CUT&Tag

Reactivity :

Human/Mouse/Rat/Other

£146.50 - £642.50

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Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MonoMethyl-Histone H3-K36 antibody (STJ23990) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-Mono-Methyl-Histone H3-K36 antibody (32-42) (STJ23990)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA/ChIP/ChIP-seq

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Western blot analysis of various lysates using MonoMethyl-Histone H3-K36 Rabbit polyclonal antibody (STJ11103389) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-Mono-Methyl-Histone H3-K36 antibody (STJ11103389)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA/ChIP-seq

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Anti-Mono-Methyl-Histone H3-K4 antibody (STJ23981)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA/ChIP/ChIP-seq

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MonoMethyl-Histone H3-K79 antibody (STJ11103474) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-Mono-Methyl-Histone H3-K79 antibody (STJ11103474)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/ChIP

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Chromatin immunoprecipitation analysis of extracts of HeLa cells, using MonoMethyl-Histone H3-K79 antibody (STJ23993) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-Mono-Methyl-Histone H3-K79 antibody (STJ23993)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA/ChIP/ChIP-seq

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Loading Control Antibodies