Western Blot Antibodies | St John's Laboratory

Western blot analysis of extracts of various cell lines, using Caspase-9 antibody (STJ113147) at 1:1000 dilution. Jurkat cells were treated by Etoposide (25 uM) at 37 °C for 5 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CASP9 antibody (250-350) (STJ113147)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Western blot analysis of various cells using Caspase-9 Polyclonal Antibody diluted at 1：500

Anti-CASP9 antibody (91-140 aa) (STJ92027)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot detection of Caspase-9 in A549, MCF7, Jurkat, K562 and Hela cell lysates using Caspase-9 mouse mAb (1:1000 diluted).Predicted band size:49/37KDa.Observed band size:49KDa.

Anti-CASP9 antibody [1D1-F2-E9] (STJ99219)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human/Transfected

€180.00 - €435.00

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Immunofluorescence analysis of Hela cell. 1, eIF2 Alpha Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Caspase 9 monoclonal antibody (3-20) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-CASP9 antibody [3-20] (STJ96979)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/IP

Reactivity :

Human/Mouse/Rat/Chicken

€60.00 - €315.00

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Anti-CASP9/Caspase 9 antibody (Lys100-Thr270) [1A127] (STJA0021960)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

Human

€286.88 - €410.63

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STJ70944 (1µg/ml) staining of MOLT4 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Caspase 3 antibody (Internal) (STJ70944)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/ICC

Reactivity :

Human/Mouse/Rat/Dog

€390.63

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STJ73021 (0. 5µg/ml) staining of Human Colon lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Caspase 6 antibody (Internal) (STJ73021)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

€390.63

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STJ73022 (0. 3µg/ml) staining of Human Colon lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-Caspase 6 antibody (Internal) (STJ73022)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

€390.63

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Western blot analysis of various lysates using Caspase-1 Rat mAb (STJ11106770) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rat IgG (H+L) (STJS000857) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Caspase-1 antibody (206-220aa aa) [SRM] (STJ11106770)

Clonality :

Monoclonal

Host :

Rat

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

€161.88 - €718.13

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Anti-caspase-1 antibody (C-Term) (STJ16101311)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P

€1,213.13

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Anti-Caspase-1 antibody [PT0628R] (STJA0039319)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€0.00

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Western blot analysis of various cells using Caspase-10 B/C Polyclonal Antibody

Anti-Caspase-10 B/C antibody (430-479 aa) (STJ92018)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€60.00 - €315.00

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Anti-Caspase-12 antibody [PT1389R] (STJA0040023)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

€0.00

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Western blot analysis of Caspase12 at dilution of 1:1000

Anti-Caspase-12 antibody [SM5104] (STJA0005104)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Mouse

€213.13 - €291.88

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Western blot analysis of lysates from Mouse kidney, using Cleaved-Caspase 3 p17 Rabbit pAb (STJ11106903) at 1:400 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 60s.

Anti-Caspase-3 antibody (119-218 aa) (STJ11106903)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Anti-Caspase-3 antibody [PT0099R] (STJA0039736)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€0.00

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Anti-caspase-4 antibody (N-Term) (STJ16101312)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P

€1,021.88

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Western blot analysis of lysates from NIH/3T3 cells, using Cleaved-Caspase 6 p18 Rabbit pAb (STJ11106914) at 1:500 dilution. NIH/3T3 cells were treated by staurosporine (1 uM) for 3 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Caspase-6 antibody (STJ11106914)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Anti-Caspase-6 antibody [PT0513R] (STJA0039200)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€0.00

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Anti-caspase-7 antibody (C-Term) (STJ16101313)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P

€1,021.88

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Anti-Caspase-7 antibody [SM0496] (STJA0010496)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

€213.13 - €291.88

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Anti-Caspase-8 antibody [PT0719R] (STJA0039206)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€0.00

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Western blot analysis of lysates from Jurkat cells, using Cleaved Caspase-9 P37 Rabbit pAb (STJ11106883) at 1:900 dilution. Jurkat cells were treated by Etoposide (25 uM) at 37 °C for 5 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-Caspase-9 antibody (250-350 aa) (STJ11106883)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Anti-Caspase-9 antibody [PT0299R] (STJA0039678)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€0.00

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Western blot analysis of Caspase-9 at dilution of 1:200

Anti-Caspase-9 antibody [SM4940] (STJA0004940)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Mouse

€213.13 - €291.88

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Anti-CASPR/Neurexin IV antibody [K65/31] (STJA0020933)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

€426.88 - €1,809.38

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Anti-CASPR/Neurexin IV antibody [K65/35] (STJA0020934)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

€426.88 - €1,809.38

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Anti-CASPR/Neurexin IV antibody [K65/36] (STJA0020935)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

€426.88 - €1,809.38

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Western blot analysis of extracts of U-87MG cells, using Caspr11 Rabbit monoclonal antibody (STJ11103532) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-Caspr1 antibody (700-800) [S2MR] (STJ11103532)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€161.88 - €718.13

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Anti-CASPR2 antibody [K67/11] (STJA0021481)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

€426.88 - €1,809.38

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Anti-CASPR2 antibody [K67/21] (STJA0021482)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

€426.88 - €1,809.38

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Anti-CASPR2 antibody [K67/25] (STJA0021483)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

€426.88 - €1,809.38

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Western blot analysis of extracts of various cell lines, using Caspr2/CNTNAP2 antibody (STJ11102993) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-Caspr2/CNTNAP2 antibody (1232-1331) [S3MR] (STJ11102993)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

€161.88 - €718.13

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Western blot analysis of lysates from THP-1 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-CASQ1 antibody (322-372 aa) (STJ194071)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunohistochemistry analysis of CASQ2 in paraffin-embedded mouse heart using CASQ2 Rabbit polyclonal antibody (STJ119183) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CASQ2 antibody (20-399) (STJ119183)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Mouse/Rat

€140.63 - €655.63

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Anti-CASQ2 antibody (STJA0007071)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Immunohistochemistry analysis of paraffin-embedded rat kidney using CASR antibody (STJ22909) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CASR antibody (180-536) (STJ22909)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Anti-CASR antibody (20-300 aa) (STJ11106237)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

€140.63 - €655.63

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Immunohistochemistry with Anti-CASR Antibody (STJ500366) at 1:50 dilution in Buffer. DAB (brown) staining and Haematoxylin QS (blue) counterstain. 40X magnification. FFPE section.

Anti-CASR antibody (47-69) (STJ500366)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€534.38

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Anti-CASR antibody (47-69) {Biotin} (STJ500367)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Anti-CASR antibody (47-69) {FITC} (STJ500368)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

€660.63

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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Calcium Sensing Receptor Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-CASR antibody (854-903 aa) (STJ92031)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€60.00 - €315.00

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Western blot analysis of lysates from rat brain, using CASR Rabbit pAb (STJ114969) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-CASR antibody (874-1088) (STJ114969)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Rat

€140.63 - €655.63

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Anti-CaSR antibody (N-Term) (STJ13100918)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

€414.38

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Western blot of lysed HFF cells that were serum starved for 24 hours and then refed for 30 minutes showing specific immunolabeling of the ~130-260 kDa CaSR protein.

Anti-CaSR antibody (N-Term) (STJA0003868)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

€568.13

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Anti-CaSR antibody (STJ13100917)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

€414.38

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Anti-CASS4 antibody (STJ11106870)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

€140.63 - €655.63

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Immunohistochemistry analysis of CAST in paraffin-embedded human uterine cancer using CAST Rabbit polyclonal antibody (STJ119179) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-CAST antibody (1-350) (STJ119179)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

€140.63 - €655.63

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