Immunohistochemistry Antibodies | St John's Laboratory

Western blot analysis of HeLa, using EIF3F rabbit polyclonal antibody (STJ29103) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.

Anti-EIF3F antibody (88-357) (STJ29103)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates using EIF3K Rabbit pAb (STJ112010) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-EIF3K antibody (1-218) (STJ112010)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates using EIF3L Rabbit pAb (STJ112013) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 5s.

Anti-EIF3L antibody (1-230) (STJ112013)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemistry analysis of EIF3M in paraffin-embedded rat brain using EIF3M Rabbit polyclonal antibody (STJ23513) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-EIF3M antibody (1-374) (STJ23513)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-EIF4A1 antibody [11B8] (STJ16103463)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

£746.50

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Anti-EIF4A1 antibody [3F11] (STJ16103527)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/ICC/IF/FC

Reactivity :

Human/Mouse/Rat

£746.50

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Immunohistochemical analysis of paraffin-embedded Human-colon-cancer tissue. 1, eIF4A1 monoclonal antibody (M8) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-EIF4A1 antibody [M8] (STJ97038)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-eIF4A1 antibody [SRM14653] (STJ11109383)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£0.00

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Anti-EIF4A1/EIF4A2/EIF4A3 antibody [SRM14739] (STJ11109469)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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SP1-Induced Upregulation of LncRNA AFAP1-AS1 Promotes Tumor Progression in Triple-Negative Breast Cancer by Regulating mTOR Pathway. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Yidong Zhou WB Human 1:500 MDA-231 cell, MDA-MB-468 cell

Anti-EIF4B antibody (209-259) (STJA0006725)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of A549 cells, using eIF4B Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-EIF4B antibody (388-437 aa) (STJ92880)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-eIF4B antibody [PT0748R] (STJA0039218)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£150.00 - £540.00

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Western blot analysis of various lysates, using eIF4E antibody (STJ23516) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.

Anti-EIF4E antibody (1-100) (STJ23516)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Western blot analysis of various cells using eIF4E Polyclonal Antibody diluted at 1：1000

Anti-EIF4E antibody (168-217 aa) (STJ92881)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

£49.00 - £261.00

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Western blot analysis using eIF4E monoclonal antibody against HeLa (1) , HEK293 (2) and K562 (3) cell lysate.

Anti-EIF4E antibody [5D11] (STJ98016)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human

£149.00 - £360.00

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Anti-eIF4E antibody [PT0569R] (STJA0039248)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using eIF4EBP1 antibody (STJ119085) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-EIF4EBP1 antibody (1-118) (STJ119085)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various cells using 4E-BP1 Polyclonal Antibody diluted at 1：500

Anti-EIF4EBP1 antibody (13-62 aa) (STJ91383)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Sheep

£49.00 - £261.00

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Immunofluorescence analysis of A549 cells, using 4E-BP1 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-EIF4EBP1 antibody (2-51 aa) (STJ91387)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using 4E-BP1 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-EIF4EBP1 antibody (30-79 aa) (STJ91384)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using 4E-BP1 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-EIF4EBP1 antibody (4-53 aa) (STJ91386)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using eIF4EBP1 antibody (STJ29842) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-EIF4EBP1 antibody (50-118) (STJ29842)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis using 4E-BP1 monoclonal antibody against truncated 4E-BP1 recombinant protein (1).

Anti-EIF4EBP1 antibody [11G12C11] (STJ97805)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human

£144.00 - £348.00

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Anti-eIF4EBP1 antibody [SRM14766] (STJ11109496)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-eIF4G antibody [PT1373R] (STJA0040018)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Immunofluorescence analysis of HeLa cells, using eIF4G Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-EIF4G1 antibody (1074-1123 aa) (STJ92883)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of extracts of HeLa cells, using EIF4G1 antibody (STJ29690) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-EIF4G1 antibody (400-500) (STJ29690)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human

£110.50 - £505.50

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Anti-EIF4G1 antibody (551-650 aa) [SRM5271] (STJ11107173)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Mouse/Rat

£126.50 - £554.50

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g EIF4G2/p97 antibody (STJ23521). Western blot was performed from the immunoprecipitate using EIF4G2/p97 antibody (STJ23521) at a dilution of 1:1000.

Anti-EIF4G2 antibody (320-490) (STJ23521)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various cells using DAP-5 Polyclonal Antibody

Anti-EIF4G2 antibody (41-90 aa) (STJ92651)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-EIF4G2/p97 antibody [SRM14441] (STJ11109171)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4°C overnight. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-EIF5 antibody (Internal) (STJ92884)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Immunofluorescence analysis of U2OS cells using eIF5A antibody (STJ23522). Blue: DAPI for nuclear staining.

Anti-EIF5A antibody (1-154) (STJ23522)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Immunohistochemistry analysis of paraffin-embedded Human breast cancer using eIF5A antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-EIF5A antibody (1-19 aa) [R09-3F9] (STJA0010890)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse/Rat

£246.50

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STJ72008 (0. 1µg/ml) staining of MOLT4 lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-eIF5A antibody (Internal) (STJ72008)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human

£312.50

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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-EIF5B antibody (1051-1100 aa) (STJ92886)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-Eimeria tenella Membrane-associated calcum-binding protein antibody (Gly24-Leu304) [1A148] (STJA0021973)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

E.tenella

£211.50 - £290.50

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Anti-Eimeria tenella Microneme protein antibody (Glu253-Val482) [1A149] (STJA0021974)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

E.tenella

£211.50 - £290.50

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Anti-ELAC1 antibody (STJA0004778)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/ELISA

Reactivity :

Human/Mouse

£139.50 - £403.50

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Anti-ELAC1 antibody (STJA0004779)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/ELISA

Reactivity :

Human/Mouse

£139.50 - £403.50

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Immunohistochemistry analysis of paraffin-embedded mouse kidney using ELAC2 antibody (STJ29208) at dilution of 1:200 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ELAC2 antibody (1-270) (STJ29208)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemistry analysis of paraffin-embedded human brain, using ELAC2 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-ELAC2 antibody (161-210 aa) (STJ95517)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of THP-1, using Neutrophil Elastase (ELANE) rabbit polyclonal antibody (STJ114982) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-ELANE antibody (30-267) (STJ114982)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-Elastase antibody [265-3K1] (STJ16100780)

Clonality :

Monoclonal

Host :

Mouse

Applications :

FC/ELISA/IF/IHC-P/WB

Reactivity :

Human

£757.50

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Immunofluorescence analysis of U2OS cells using HuR/ELAVL1 antibody (STJ116295) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-ELAVL1 antibody (1-100) (STJ116295)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse

£126.50 - £618.50

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Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g HuR/ELAVL1 antibody (STJ11101733). Western blot was performed from the immunoprecipitate using HuR/ELAVL1 antibody (STJ11101733) at a dilution of 1:1000.

Anti-ELAVL1 antibody (1-100) [S3MR] (STJ11101733)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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Immunofluorescence analysis of MCF-7 cells using HuR/ELAVL1 antibody (STJ23524). Blue: DAPI for nuclear staining.

Anti-ELAVL1 antibody (1-326) (STJ23524)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Anti-ELAVL1 antibody [PT1472R] (STJA0040284)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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