Immunohistochemistry Antibodies | St John's Laboratory

Western blot analysis of extracts of various cell lines, using Prohibitin antibody (STJ11101679) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-PHB antibody (100-200) [S9MR] (STJ11101679)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€142.20 - €625.80

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Immunohistochemistry analysis of paraffin-embedded human lung cancer using Prohibitin Rabbit polyclonal antibody (STJ115487) at dilution of 1:500 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-PHB antibody (73-272) (STJ115487)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€132.60 - €606.60

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Western blot analysis of extracts of various cell lines, using Prohibitin rabbit polyclonal antibody antibody (STJ24977) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PHB antibody (73-272) (STJ24977)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€132.60 - €606.60

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Western blot analysis using Prohibitin monoclonal antibody against A431 (1) , MCF-7 (2) , Jurkat (3) , HeLa (4) , HepG2 (5) , A549 (6) , NIH/3T3 (7) , Cos7 (8) and PC-12 (9) cell lysate.

Anti-PHB1 antibody [5H7] (STJ98339)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/FC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€178.80 - €432.00

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Western blot analysis of various cells using PHF1 Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-PHF1 antibody (345-394 aa) (STJ95062)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of various cells using PHLDA3 Polyclonal Antibody

Anti-PHLDA3 antibody (21-70 aa) (STJ95068)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Immunofluorescence analysis of rat-liver tissue. 1, Akt (phospho Ser473) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-Akt-Ser473 antibody (431-480 aa) (STJ90166)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Chicken

€58.80 - €313.20

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Anti-Phospho-AKT-Ser473 antibody [R06-7C5] (STJA0036228)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

€160.20 - €337.80

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Immunofluorescence analysis of human-lung tissue. 1, Akt (phospho Thr308) Polyclonal Antibody (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-Phospho-Akt-Thr308 antibody (STJ90750)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/D.melanogaster

€58.80 - €313.20

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Immunohistochemistry analysis of paraffin-embedded human brain, using AKT1/2/3 (Phospho-Tyr315/316/312) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-Akt-Tyr315 antibody (281-330 aa) (STJ91195)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Anti-Phospho-AKT1-S212 antibody [SAA0279] (STJA0019075)

Clonality :

Monoclonal

Host :

Rat

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse

€234.60 - €1,583.40

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Immunohistochemical analysis of paraffin-embedded Human Lung Carcinoma Tissue using Phospho-Akt Ser473 Mouse mAb diluted at 1:200.

Anti-Phospho-AKT1-S473 antibody (410-490 aa) [7F9] (STJ97727)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF

Reactivity :

Human

€58.80 - €313.20

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Western blot analysis of lysates from Jurka cells, using Phospho-AKT1-S473 Rabbit pAb (STJ22011) at 1:1000 dilution. Jurkat cells were treated by Serum-starvation overnight at 37 °C. Jurkat cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-AKT1-S473 antibody (STJ22011)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Anti-Phospho-AKT1-S473 antibody [P8H9] (STJA0019074)

Clonality :

Monoclonal

Host :

Rat

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat/AKT8 murine leukemia virus

€234.60 - €1,583.40

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Western blot analysis of various lysates using Phospho-AKT1-S473 mAb (STJ113518) at 1:1000 dilution. Both Jurkat cells and C6 cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-AKT1-S473 antibody [S8MR] (STJ113518)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€172.20 - €742.20

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Anti-Phospho-AKT1-S473 antibody [SAA0300] (STJA0019165)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IP/WB

Reactivity :

Human

€234.60 - €1,583.40

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Western blot analysis of mouse cells using Phospho-Akt (S124) Polyclonal Antibody

Anti-Phospho-AKT1-Ser124 antibody (90-139 aa) (STJ90781)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of various cells using Phospho-Akt1 (S246) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-AKT1-Ser246 antibody (212-261 aa) (STJ90165)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of lysates from HeLa cells, using Phospho-AKT1-T308 Rabbit pAb (STJ11103747) at 1:1000 dilution. HeLa cells were treated by PDGF (100 ng/ml) at 37 °C for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-AKT1-T308 antibody (STJ11103747)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Immunohistochemistry analysis of paraffin-embedded human kidney using Phospho-Akt-T308 Rabbit monoclonal antibody (STJ11105823) at dilution of 1:60 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Phospho-AKT1-T308 antibody [S5823RM] (STJ11105823)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€142.20 - €625.80

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Western blot analysis of various cells using Phospho-Akt1 (T450) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-AKT1-Thr450 antibody (416-465 aa) (STJ90168)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Anti-Phospho-AKT1-Thr450 antibody [R72-1U-9] (STJA0035669)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse/Rat

€160.20 - €337.80

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Western blot analysis of 3T3 cells using Phospho-Akt1 (T72) Polyclonal Antibody

Anti-Phospho-AKT1-Thr72 antibody (38-87 aa) (STJ90844)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of various cells using Phospho-Akt1 (Y474) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-AKT1-Tyr474 antibody (431-480 aa) (STJ90843)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€58.80 - €313.20

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Western blot analysis of various cells using Phospho-Akt1/3 (Y437/434) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-Akt1/3-Tyr437/434 antibody (406-455 aa) (STJ90938)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Immunofluorescence analysis of Hela cell. 1, AMPK Alpha 1/2 (phospho Thr183/172) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-AMPK Alpha 1/2-Thr183/172 antibody (140-189 aa) (STJ90735)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat/Monkey/Pig/M.japonicus

€58.80 - €313.20

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Immunohistochemistry analysis of paraffin-embedded human brain, using AurB/C (Phospho-Thr236/202) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ARK-2/3-Thr236/202 antibody (201-250 aa) (STJ91175)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western Blot analysis of 1 K562 cell, 2 LPS 100ng/mL 30min treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-ATM-Ser179 antibody (STJA0010053)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

€58.80 - €313.20

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Western blot analysis of K562 using p-Atm (S1981) antibody. Antibody was diluted at 1:500

Anti-Phospho-ATM-Ser1981 antibody (STJ97691)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Anti-Phospho-ATM-Ser1981 antibody [PT0902R] (STJA0039795)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€180.00 - €648.00

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Anti-Phospho-ATM-Ser1981 antibody [R01-5I8] (STJA0035784)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ICC/IF/IP

Reactivity :

Human

€160.20 - €337.80

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Western Blot analysis of mouse heart, Hela, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-ATM-Ser367 antibody (STJA0010035)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

€58.80 - €313.20

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Western blot analysis of lysates from 293T cells, using Phospho-ATR-S428 Rabbit pAb (STJ114848) at 1:400 dilution. 293T cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-ATR-S428 antibody (STJ114848)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Anti-Phospho-ATR-S428-S428 antibody [SRM15157] (STJ11109887)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/IHC-P/ELISA

Reactivity :

Human

€142.20 - €625.80

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using ATR (Phospho-Ser428) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-ATR-Ser428 antibody (394-443 aa) (STJ91234)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Anti-Phospho-ATR-Ser428 antibody [PT1243R] (STJA0040115)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€180.00 - €648.00

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Western blot analysis of lysates from HeLa cells, using Phospho-ATR-T1989 Rabbit pAb (STJ11103765) at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-ATR-T1989 antibody (STJ11103765)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using AurA (Phospho-Ser342) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-AURKA-Ser342 antibody (311-360 aa) (STJ91174)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of 293 cells using Phospho-ARK-1 (T288) Polyclonal Antibody

Anti-Phospho-AURKA-Thr288 antibody (256-305 aa) (STJ90805)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Immunohistochemistry analysis of paraffin-embedded human liver carcinoma, using AurB (Phospho-Thr232) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-AURKB-Thr232 antibody (198-247 aa) (STJ91089)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€58.80 - €313.20

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Western blot analysis of lysates from K562 cells, using AurB (Phospho-Tyr12) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-AURKB-Tyr12 antibody (1-50 aa) (STJ90920)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western Blot analysis of 1 Raji cell, 2 Serum-free treated, using primary antibody at 1:1000 dilution. Secondary antibody (STJS000791) was diluted at 1:10000

Anti-Phospho-BCL6-Ser343 antibody (STJA0010020)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human/Mouse

€58.80 - €313.20

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Western blot analysis of lysates from HeLa cells, using Phospho-CDK1-T161 Rabbit pAb (STJ22061) at 1:400 dilution. HeLa cells were treated by EGF. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-CDK1-T161 antibody (STJ22061)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Anti-Phospho-CDK1-T161+CDK2/CDK3-T160-T161+T160 antibody [SRM15146] (STJ11109876)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€142.20 - €625.80

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-CDK1-Thr161 antibody (126-175 aa) (STJ91199)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of various cells using Phospho-Cdc2 (T161) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-CDK1-Thr161 antibody (131-180 aa) (STJ90213)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of lysates from HeLa cells, using Phospho-CDK1-Y15 Rabbit pAb (STJ23048). HeLa cells were treated by nocodazole (50 ng/mL) at 37 °C for 20 hours or Hydroxyurea (4 mM) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-CDK1-Y15 antibody (STJ22059)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Western blot analysis of lysates from C6 cells, using Phospho-CDK2-T160 Rabbit pAb (STJ22062) at 1:400 dilution. C6 cells were treated by Nocodazole (10 ng/ml) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-CDK2-T160 antibody (STJ22062)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

Choose Options