Immunofluorescence Antibodies | St John's Laboratory

Western blot analysis of lysates from HeLa cells, using Phospho-CDK1-T161 Rabbit pAb (STJ22061) at 1:400 dilution. HeLa cells were treated by EGF. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-Phospho-CDK1-T161 antibody (STJ22061)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Anti-Phospho-CDK1-T161+CDK2/CDK3-T160-T161+T160 antibody [SRM15146] (STJ11109876)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

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Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-CDK1-Thr161 antibody (126-175 aa) (STJ91199)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of various cells using Phospho-Cdc2 (T161) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-CDK1-Thr161 antibody (131-180 aa) (STJ90213)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of lysates from HeLa cells, using Phospho-CDK1-Y15 Rabbit pAb (STJ23048). HeLa cells were treated by nocodazole (50 ng/mL) at 37 °C for 20 hours or Hydroxyurea (4 mM) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-CDK1-Y15 antibody (STJ22059)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Anti-Phospho-CDK1/2-Thr14 antibody [PT0910R] (STJA0039801)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€180.00 - €648.00

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Western blot analysis of various cells using Phospho-Cdk1/2/3 (T14) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-Cdk1/2/3-Thr14 antibody (1-50 aa) (STJ90219)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€58.80 - €313.20

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Anti-Phospho-CDK2-T160-T160 antibody [SRM15122] (STJ11109852)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF-P/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

€0.00

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Western blot analysis of various cells using Phospho-Cdk2/Cdc2 (T160) Polyclonal Antibody diluted at 1：500

Anti-Phospho-CDK2-Thr160 antibody (STJ90752)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-CDK4-T172 Rabbit pAb (STJ23055). NIH/3T3 cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-CDK4-T172 antibody (STJ29359)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Western blot analysis of HT29+PMA cells using Phospho-Cdk5 (Y15) Polyclonal Antibody

Anti-Phospho-CDK5-Tyr15 antibody (1-50 aa) (STJ90540)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€58.80 - €313.20

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Western blot analysis of lysates from 293 cells using Phospho-CDK6-Y13 Rabbit pAb (STJ22064). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-CDK6-Y13 antibody (STJ22064)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

€151.80 - €742.20

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Western blot analysis of lysates from 293 cells using Phospho-CDK6-Y24 Rabbit pAb (STJ22063). <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-CDK6-Y24 antibody (STJ22063)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Human/Mouse

€151.80 - €742.20

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CDK7 (Phospho-Thr170) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CDK7-Thr170 antibody (136-185 aa) (STJ90891)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€58.80 - €313.20

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Western blot analysis of various cells using Phospho-p21 (T145) Polyclonal Antibody

Anti-Phospho-CDKN1A-Thr145 antibody (111-160 aa) (STJ90360)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Anti-Phospho-CDKN1B-Ser178 antibody (144-193 aa) (STJ91151)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse

€58.80 - €313.20

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Anti-Phospho-CDKN1B-Thr157 antibody (123-172 aa) (STJ91150)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Western blot analysis of various cells using Phospho-p27 (T187) Polyclonal Antibody

Anti-Phospho-CDKN1B-Thr187 antibody (149-198 aa) (STJ90443)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using p57 Kip2 (Phospho-Thr310) Antibody

Anti-Phospho-CDKN1C-Thr310 antibody (267-316 aa) (STJ91087)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€58.80 - €313.20

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Western blot analysis of various cells using Phospho-C/EBP Alpha (S21) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-CEBPA-Ser21 antibody (6-55 aa) (STJ90757)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of various cells using Phospho-C/EBP Beta (T235) Polyclonal Antibody diluted at 1：500

Anti-Phospho-CEBPB-Thr235 antibody (201-250 aa) (STJ90200)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Immunohistochemistry analysis of paraffin-embedded human brain, using C/EBP-epsilon (Phospho-Thr74) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CEBPE-Thr74 antibody (40-89 aa) (STJ91071)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Immunofluorescence analysis of HeLa cells, using Centromeric Protein A (Phospho-Ser7) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CENPA-Ser7 antibody (1-50 aa) (STJ91332)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/ELISA

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-Phospho-CEP55-Ser425 antibody (STJ91247)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Western blot analysis of lysates from NIH/3T3 cells, using Phospho-CFL1-S3 Rabbit pAb (STJ26176). NIH/3T3 cells were treated by CIP (20uL/400ul) at 37 °C for 1 hour. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-Phospho-CFL1-S3 antibody (STJ22068)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Western blot analysis of NIH-3T3 cells using Phospho-Cofilin (S3) Polyclonal Antibody

Anti-Phospho-CFL1-Ser3 antibody (1-50 aa) (STJ90230)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Anti-Phospho-CFTR-Ser737 antibody (STJ193221)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of lysates from K562 cells treated with Na3VO4 0.3uM 40', using Chk1 (Phospho-Ser286) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-CHEK1-Ser286 antibody (256-305 aa) (STJ90705)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Western blot analysis of lysates from HUVEC cells treated with UV 15', using Chk1 (Phospho-Ser296) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-CHEK1-Ser296 antibody (266-315 aa) (STJ90849)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Western blot analysis of 293T cells using Phospho-Chk1 (S301) Polyclonal Antibody diluted at 1：2000

Anti-Phospho-CHEK1-Ser301 antibody (271-320 aa) (STJ90706)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of 293T cells using Phospho-Chk1 (S317) Polyclonal Antibody diluted at 1：500

Anti-Phospho-CHEK1-Ser317 antibody (286-335 aa) (STJ90222)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Chk1 (Phospho-Ser345) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CHEK1-Ser345 antibody (310-359 aa) (STJ90223)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of Hela cells using Phospho-Chk2 (T383) Polyclonal Antibody diluted at 1：1000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-CHEK2-Thr383 antibody (356-405 aa) (STJ90698)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

€58.80 - €313.20

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Western blot analysis of Hela cells using Phospho-Chk2 (T387) Polyclonal Antibody diluted at 1：500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-CHEK2-Thr387 antibody (361-410 aa) (STJ90784)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Immunofluorescence analysis of Hela cell. 1, Chk2 (phospho Thr68) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). HAO1 monoclonal antibody (Mix) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-CHEK2-Thr68 antibody (35-84 aa) (STJ90225)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Anti-Phospho-Chk1-Ser317 antibody [PT0744R] (STJA0040059)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€180.00 - €648.00

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Anti-Phospho-Chk1-Ser345 antibody [PT0651R] (STJA0039191)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

€342.00 - €648.00

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Anti-Phospho-Chk2-Thr68 antibody [PT0729R] (STJA0039130)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€180.00 - €648.00

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Western blot analysis of various cells using Phospho-IKK Alpha (T23) Polyclonal Antibody diluted at 1：1000

Anti-Phospho-CHUK-Thr23 antibody (15-64 aa) (STJ90300)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of various lysates using Phospho-c-Jun-T91 Rabbit pAb (STJ11103749) at 1:1000 dilution. Both 293T cells and NIH/3T3 cells were treated by Anisomycin (25 Mu g/mL) at 37 °C for 30 minutes after serum-starvation overnight. Both 293T cells and NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 180s.

Anti-Phospho-cJun-T91 antibody (STJ11103749)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

€151.80 - €742.20

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Western blot analysis of lysates from COLO205 cells treated with EGF 200ng/ml 30', using Claudin 3 (Phospho-Tyr219) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-CLDN3-Tyr219 antibody (171-220 aa) (STJ90624)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Anti-Phospho-CLDN4-Tyr208 antibody (160-209 aa) (STJ91248)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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Immunohistochemistry analysis of paraffin-embedded human brain, using Claudin 5 (Phospho-Tyr217) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CLDN5-Tyr217 antibody (169-218 aa) (STJ91249)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Western blot analysis of lysates from RAW264.7 cells treated with UV 5', using Claudin 6 (Phospho-Tyr219) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-Phospho-CLDN6-Tyr219 antibody (171-220 aa) (STJ90625)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

€58.80 - €313.20

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Immunohistochemistry analysis of paraffin-embedded human brain, using CNOT2 (Phospho-Ser101) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-CNOT2-Ser101 antibody (67-116 aa) (STJ91265)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

€58.80 - €313.20

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Anti-Phospho-Cofilin-Ser3 antibody [PT0749R] (STJA0040280)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

€180.00 - €648.00

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Anti-Phospho-CREB-1-Ser133 antibody [PT0863R] (STJA0039162)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/IP/ELISA

Reactivity :

Human/Mouse/Rat

€180.00 - €648.00

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Anti-Phospho-CREB-2-Ser245 antibody (212-261) (STJA0039125)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF-P/ELISA

Reactivity :

Human/Rat/Mouse

€58.80 - €313.20

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