ELISA Antibodies | St John's Laboratory

Whole cell lysates were separated by 8% SDS-PAGE, and the membrane was blotted with anti-CD27 (PT1818) antibody. The HRP-conjugated anti- mouse IgG antibody was used to detect the antibody. Lane 1: K562Predicted band size: 29kDaObserved band size: 40kDa

Anti-CD27 antibody (160-260) [PT1818] (STJA0006232)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£49.00 - £261.00

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STJ73070 (0. 3µg/ml) staining of Human Spleen (A) , Tonsil (B) and Thymus (C) lysates (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CD27 antibody (161-175) (STJ73070)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

£312.50

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Western blot analysis of lysates from Mouse lung, using CD27 Rabbit polyclonal antibody (STJ118100) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CD27 antibody (20-191) (STJ118100)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£110.50 - £505.50

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Western blot analysis of extracts of Raji cells, using CD27 antibody (STJ22984) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-CD27 antibody (20-191) (STJ22984)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-CD27 antibody [PT1331R] (STJA0040116)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£150.00 - £540.00

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Immunohistochemistry analysis of CD271 in paraffin-embedded Human tonsil tissue using CD271 Rabbit pAb (STJ11107099) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0. 01M Citrate Buffer (pH 6. 0) prior to immunohistochemistry staining.

Anti-CD271 antibody (32-254 aa) (STJ11107099)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC-P/ELISA

Reactivity :

Human

£110.50 - £505.50

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Flow cytometry:1X10^6 293F (Transfection) cells cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (2 Mu g/mL, left) or CD271/NGFR Rabbit monoclonal antibody (STJ11105254, 2 Mu g/mL, right).

Anti-CD271 antibody (32-254) [S5254RM] (STJ11105254)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/FC/ELISA

Reactivity :

Mouse/Rat

£126.50 - £554.50

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Anti-CD271/NGFR antibody [T-2] (STJA0027783)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£254.50 - £329.50

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Anti-CD271/NGFR antibody [T-2] {APC} (STJA0029063)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£310.50 - £405.50

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Anti-CD271/NGFR antibody [T-2] {FITC} (STJA0028097)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£254.50 - £329.50

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Anti-CD271/NGFR antibody [T-2] {PerCP} (STJA0030029)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£292.50 - £379.50

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Anti-CD271/NGFR antibody [T-2] {PE} (STJA0030995)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Human

£310.50 - £405.50

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Anti-CD272/BTLA antibody [SRM13405] (STJ11108135)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/FC/ELISA

Reactivity :

Mouse

£0.00

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Anti-CD273/PD-L2/PDCD1LG2 nanobody [SAA1281] (STJN000298)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA/FC/SPR

Reactivity :

Human

£209.50 - £1,333.50

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Immunohistochemical analysis of paraffin-embedded Human-tonsil, antibody was diluted at 1:100

Anti-CD274 antibody (181-230 aa) (STJ98996)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£49.00 - £261.00

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Western blot analysis of lysates from Mouse thymus, using CD274 Rabbit monoclonal antibody (STJ11105258) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-CD274 antibody (19-235) [S5258RM] (STJ11105258)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse

£118.50 - £521.50

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Immunohistochemistry analysis of PD-L1/CD274 in paraffin-embedded human placenta using PD-L1/CD274 Rabbit polyclonal antibody (STJ113753) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CD274 antibody (19-238) (STJ113753)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using CD274 antibody (STJ11103333) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CD274 antibody (19-239) (STJ11103333)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Immunofluorescence analysis of paraffin-embedded human placenta using PD-L1/CD274 Rabbit polyclonal antibody (STJ22985) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CD274 antibody (190-290) (STJ22985)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemistry of baboon spleen with Anti-CD274 primary antibody (STJ502399) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-CD274 antibody (80-130) (STJ502399)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£427.50

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Anti-CD274 antibody (80-130) {Biotin} (STJ502400)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£528.50

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Anti-CD274 antibody (80-130) {FITC} (STJ502401)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£528.50

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Immunohistochemistry analysis of PD-L1/CD274 in paraffin-embedded human placenta using PD-L1/CD274/CD274 Rabbit monoclonal antibody (STJ11103966) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CD274 antibody (ECD) [S3966RM] (STJ11103966)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£126.50 - £554.50

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Anti-CD274 antibody [SRM15057] (STJ11109787)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IHC-P/FC/ELISA

Reactivity :

Mouse

£0.00

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STJ70646 (0. 01µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-CD274/PD-L1 antibody (C-Term) (STJ70646)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human

£312.50

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Biotinylated STJ70646 (0. 3µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-CD274/PD-L1, Biotinylated antibody (C-Term) {Biotin} (STJ73200)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

£312.50

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Anti-CD274/PD-L1/B7-H1 antibody (Met1-Arg238) [1A058] (STJA0021896)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

Human

£211.50 - £290.50

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Anti-CD274/PD-L1/B7-H1 antibody (Met1-Arg238) [1A059] (STJA0021897)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

Human

£211.50 - £290.50

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Anti-CD274/PD-L1/B7-H1 antibody (Met1-Arg238) [1A060] (STJA0021898)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

Human

£211.50 - £290.50

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Anti-CD274/PD-L1/B7-H1 antibody (Met1-Arg238) [1A061] (STJA0021899)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/IHC/WB

Reactivity :

Human

£211.50 - £290.50

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Anti-CD274/PD-L1/B7-H1 antibody [10F.9G2] (STJA0032307)

Clonality :

Monoclonal

Host :

Rat

Applications :

ELISA/FCM/IHC-F

Reactivity :

Mouse

£310.50

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Anti-CD274/PD-L1/B7-H1 antibody [27A4] (STJA0018690)

Clonality :

Monoclonal

Host :

Mouse

Applications :

Blocking/ELISA/FC

Reactivity :

Human

£209.50 - £1,333.50

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Anti-CD274/PD-L1/B7-H1 antibody [29E.2A3] (STJA0032238)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/Func

Reactivity :

Human

£310.50

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Anti-CD274/PD-L1/B7-H1 antibody [Fs0347] (STJA0032308)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FCM/Neut

Reactivity :

Mouse

£367.50

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Anti-CD274/PD-L1/B7-H1 antibody [SAA0142] (STJA0018689)

Clonality :

Monoclonal

Host :

Mouse

Applications :

Blocking/ELISA/FC

Reactivity :

Mouse

£209.50 - £1,333.50

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Anti-CD274/PD-L1/B7-H1 antibody [SAA0268] (STJA0027477)

Clonality :

Monoclonal

Host :

Rat

Applications :

ELISA/FC

Reactivity :

Mouse

£112.50 - £165.50

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Anti-CD274/PD-L1/B7-H1 antibody [SAA0268] {APC} (STJA0029702)

Clonality :

Monoclonal

Host :

Rat

Applications :

ELISA/FC

Reactivity :

Mouse

£134.50 - £196.50

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Anti-CD274/PD-L1/B7-H1 antibody [SAA0268] {FITC} (STJA0028736)

Clonality :

Monoclonal

Host :

Rat

Applications :

ELISA/FC

Reactivity :

Mouse

£130.50 - £165.50

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Anti-CD274/PD-L1/B7-H1 antibody [SAA0268] {PerCP} (STJA0030668)

Clonality :

Monoclonal

Host :

Rat

Applications :

ELISA/FC

Reactivity :

Mouse

£134.50 - £186.50

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Anti-CD274/PD-L1/B7-H1 antibody [SAA0268] {PE} (STJA0031634)

Clonality :

Monoclonal

Host :

Rat

Applications :

ELISA/FC

Reactivity :

Mouse

£130.50 - £196.50

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Anti-CD274/PD-L1/B7-H1 antibody [SAA0359] (STJA0027542)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Mouse

£231.50 - £300.50

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Anti-CD274/PD-L1/B7-H1 antibody [SAA0359] {APC} (STJA0029703)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Mouse

£282.50 - £366.50

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Anti-CD274/PD-L1/B7-H1 antibody [SAA0359] {FITC} (STJA0028737)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Mouse

£231.50 - £298.50

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Anti-CD274/PD-L1/B7-H1 antibody [SAA0359] {PerCP} (STJA0030669)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Mouse

£265.50 - £344.50

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Anti-CD274/PD-L1/B7-H1 antibody [SAA0359] {PE} (STJA0031635)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/FC

Reactivity :

Mouse

£282.50 - £366.50

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Anti-CD274/PD-L1/B7-H1 antibody [SAA2003] (STJA0020550)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/WB

Reactivity :

Human

£212.50 - £1,184.50

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Anti-CD274/PD-L1/B7-H1 antibody [SAA2018] (STJA0020565)

Clonality :

Monoclonal

Host :

Human

Applications :

ELISA/WB

Reactivity :

Human

£212.50 - £1,184.50

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Anti-CD274/PD-L1/B7-H1 nanobody [C3] (STJN000588)

Clonality :

Monoclonal

Host :

Alpaca

Applications :

ELISA/FuncS

Reactivity :

Mouse

£209.50 - £1,333.50

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