| Applications: | ELISA |
| Reactivity: | Mouse |
| Note: | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: | 1.41 pg/mL |
| Detection Limit: | 3.13-200 pg/mL |
| Short Description: | This TNF-Alpha Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma and other biological fluids. |
| Storage Instruction: | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: | 3.5h |
| Detection: | Colormetric |
| Gene Symbol: | Il1b |
| Gene ID: | 16176 |
| Uniprot ID: | IL1B_MOUSE |
| Specificity: | This kit recognizes Mouse IL-1 Beta in samples.No significant cross-reactivity or interference between Mouse IL-1 Beta and analogues was observed |
| Sample Type: | serum, plasma and other biological fluids |
| Tissue Specificity | Expressed in activated macrophages (at protein level). |
| Function | Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Involved in transduction of inflammation downstream of pyroptosis: its mature form is specifically released in the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. |
| Protein Name | Interleukin-1 Beta Il-1 Beta |
| Database Links | Reactome: R-MMU-448706 Reactome: -MMU-5620971 Reactome: -MMU-5660668 Reactome: -MMU-9020702 |
| Cellular Localisation | Cytoplasm Cytosol Secreted Lysosome Extracellular Exosome The Precursor Is Cytosolic In Response To Inflammasome-Activating Signals Such As Atp For Nlrp3 Inflammasome Or Bacterial Flagellin For Nlrc4 Inflammasome Cleaved And Secreted Mature Form Is Secreted And Released In The Extracellular Milieu By Passing Through The Gasdermin-D (Gsdmd) Pore In Contrast The Precursor Form Is Not Released Due To The Presence Of An Acidic Region That Is Proteolytically Removed By Casp1 During Maturation The Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10 |
| Alternative ELISA Names | Interleukin-1 Beta ELISA kit Il-1 Beta ELISA kit Il1b ELISA kit |
| Specificity | This kit recognizes Mouse IL-1β in samples. No significant cross-reactivity or interference between Mouse IL-1β and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 91-107 | 98 |
| EDTA plasma (n=8) | 93-110 | 100 |
| Cell culture media (n=8) | 90-104 | 97 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 9.10 | 20.17 | 95.02 | 9.50 | 21.18 | 102.03 |
| Standard deviation | 0.63 | 0.93 | 4.46 | 0.63 | 1.05 | 4.02 |
| CV (%) | 6.93 | 4.60 | 4.69 | 6.63 | 4.94 | 3.94 |
