| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
9.38pg/mL |
| Detection Limit: |
15.63~1000pg/mL |
| Short Description: |
This Tgfb2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma and other biological fluids. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Tgfb2 |
| Gene ID: |
21808 |
| Uniprot ID: |
TGFB2_MOUSE |
| Specificity: |
This kit recognizes Mouse TGF-Beta 2 in samples. No significant cross-reactivity or interference between Mouse TGF-Beta 2 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Function | Transforming growth factor beta-2 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains, which constitute the regulatory and active subunit of TGF-beta-2, respectively. Latency-associated peptide: Required to maintain the Transforming growth factor beta-2 (TGF-beta-2) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-2 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2. Transforming growth factor beta-2: Multifunctional protein that regulates various processes such as angiogenesis and heart development. Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains remain non-covalently linked rendering TGF-beta-2 inactive during storage in extracellular matrix. At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2 and maintain it in a latent state during storage in extracellular milieus. Once activated following release of LAP, TGF-beta-2 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal. |
| Protein Name | Transforming Growth Factor Beta-2 Proprotein Cleaved Into - Latency-Associated PeptideLap - Transforming Growth Factor Beta-2Tgf-Beta-2 |
| Database Links | Reactome: R-MMU-114608Reactome: -MMU-2129379Reactome: -MMU-2173789Reactome: -MMU-9839389 |
| Cellular Localisation | Latency-Associated Peptide: SecretedExtracellular SpaceExtracellular MatrixTransforming Growth Factor Beta-2: Secreted |
| Alternative ELISA Names | Transforming Growth Factor Beta-2 Proprotein Cleaved Into - Latency-Associated Peptide ELISA kitLap - Transforming Growth Factor Beta-2 ELISA kitTgf-Beta-2 ELISA kitTgfb2 ELISA kit |
| Specificity | This kit recognizes Mouse TGF-β2 in samples. No significant cross-reactivity or interference between Mouse TGF-β2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 94-106 | 99 |
| EDTA plasma (n=8) | 87-99 | 93 |
| Cell culture media (n=8) | 88-99 | 94 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 51.37 | 84.50 | 499.92 | 49.73 | 76.67 | 544.03 |
| Standard deviation | 2.76 | 4.53 | 21.85 | 2.63 | 3.98 | 20.13 |
| CV (%) | 5.37 | 5.36 | 4.37 | 5.29 | 5.19 | 3.70 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
