| Short Description : | Recombinant-Mouse Sepiapterin reductase/SPR-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications: | SDS-PAGE |
| Host: | E.coli |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Concentration: | 1 mg/mL |
| Formulation: | Liquid in 20mM Tris-HCl buffer (pH 8.5) containing 1mM DTT, 10% Glycerol |
| Storage Instruction: | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Accession Number: | NP_035597 |
| Immunogen: | Mouse |
| Immunogen Region: | 1-262aa |
| Immunogen Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMEAGGLG CAVCVLTGAS RGFGRALAPQ LARLLSPGSV MLVSARSESM LRQLKEELGA QQPDLKVVLA AADLGTEAGV QRLLSAVREL PRPEGLQRLL LINNAATLGD VSKGFLNVND LAEVNNYWAL NLTSMLCLTS GTLNAFQDSP GLSKTVVNIS SLCALQPYKG WGLYCAGKAA RDMLYQVLAA EEPSVRVLSY APGPLDND |
| Background | Spr also known as Sepiapterin reductase, belongs to the Short-chain dehydrogenase/reductase (Sdr) family and also reduces various exogenous carbonyl compounds including phenylpropanedione. Spr is an essential enzyme for the biosynthesis of tetrahydrobiopterin, an essential cofactor for aromatic amino acid hydrolases including tyrosine hydroxylase, the rate-limiting enzyme in dopamine synthesis. Defects in Spr cause DOPA-responsive dystonia defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Recombinant mouse Spr protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography. |
Information sourced from Uniprot.org
