Mouse Dihydrofolate reductase/DHFR protein (Recombinant) (His-Tag) (STJP017050)
SPECIFICATIONS
HostE.coli
ImmunogenMouse
General Information
| Short Description | Recombinant-Mouse Dihydrofolate reductase/DHFR-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE/Enzyme Activity |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 1 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 10% Glycerol, 2mM DTT, 0.1M NaCl. |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Endotoxin | < 1 EU per 1ug of protein (determined by LAL method) |
| Immunoreactivity | Specific activity is > 2000pmol/min/ug, and is defined as the amount of enzyme that converts 1.0pmole of dihydrofolic acid to tetrahydrofolic acid per minute at pH 6.5 at 25C. |
Target Information
| Gene Symbol | Dhfr |
| Gene ID | 13361 |
| Uniprot ID | DYR_MOUSE |
| Accession Number | NP_034179.1 |
| Immunogen | Mouse |
| Immunogen Region | 1-187aa |
| Immunogen Sequence |
Additional Info
| Function | Key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. Binds its own mRNA. |
| Protein Name | Dihydrofolate Reductase |
| Database Links | Reactome: R-MMU-196757 |
| Cellular Localisation | MitochondrionCytoplasm |
| Alternative Protein Names | Dihydrofolate Reductase proteinDhfr protein |
Information sourced from Uniprot.org