| Applications: |
ELISA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
0.09ng/mL |
| Detection Limit: |
0.16~10ng/mL |
| Short Description: |
This Prf1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin). |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Colormetric |
| Gene Symbol: |
Prf1 |
| Gene ID: |
18646 |
| Uniprot ID: |
PERF_MOUSE |
| Specificity: |
This kit recognizes Mouse PRF1 in samples. No significant cross-reactivity or interference between Mouse PRF1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Post Translational Modifications | N-glycosylated. The glycosylation sites are facing the interior of the pore. |
| Function | Pore-forming protein that plays a key role in granzyme-mediated programmed cell death, and in defense against virus-infected or neoplastic cells. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by mediating the passage and uptake of cytotoxic granzymes. Facilitates the delivery of cationic cargo protein, while anionic or neural proteins are not delivered efficiently. Perforin pores allow the release of mature caspase-7 (CASP7) into the extracellular milieu. |
| Protein Name | Perforin-1P1CytolysinLymphocyte Pore-Forming Protein |
| Cellular Localisation | Cytolytic GranuleSecretedCell MembraneMulti-Pass Membrane ProteinEndosome LumenReleased From Cytotoxic LymphocytesTogether With Proapoptotic Granzymes: Stored In Cytolytic Granules Of Cytolytic T-Lymphocytes And Secreted Into The Cleft Between T-Lymphocyte And Target CellMay Be Taken Up Via Endocytosis Involving Clathrin-Coated Vesicles And Accumulate In A First Time In Large Early EndosomesInserts Into The Cell Membrane Of Target Cells And Forms PoresMembrane Insertion And Pore Formation Requires A Major Conformation Change |
| Alternative ELISA Names | Perforin-1 ELISA kitP1 ELISA kitCytolysin ELISA kitLymphocyte Pore-Forming Protein ELISA kitPrf1 ELISA kitPfp ELISA kit |
| Specificity | This kit recognizes Mouse PRF1 in samples. No significant cross-reactivity or interference between Mouse PRF1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 89-100 | 95 |
| EDTA plasma (n=8) | 93-106 | 99 |
| Cell culture media (n=8) | 93-108 | 99 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 0.48 | 1.22 | 4.57 | 0.49 | 1.17 | 4.45 |
| Standard deviation | 0.03 | 0.07 | 0.22 | 0.03 | 0.05 | 0.21 |
| CV (%) | 6.25 | 5.74 | 4.81 | 6.12 | 4.27 | 4.72 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
