Mouse NG2 (NG2) Antibody Pair (STJAP00801)
General Information
| Short Description | This NG2 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R53-2Z-1/R14-7S-4 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | Cspg4 |
| Gene ID | 121021 |
| Uniprot ID | CSPG4_MOUSE |
| Immunogen | Recombinant protein |
Additional Info
| Tissue Specificity | Expressed in microcascular pericytes and not endothelial cells. |
| Post Translational Modifications | O-glycosylated.contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation. Involved in interaction with MMP16 and ITGA4. Phosphorylation by PRKCA regulates its subcellular location and function in cell motility. |
| Function | Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Also functions as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades. |
| Protein Name | Chondroitin Sulfate Proteoglycan 4Chondroitin Sulfate Proteoglycan Ng2Proteoglycan An2 |
| Database Links | Reactome: R-MMU-1971475Reactome: -MMU-2022870Reactome: -MMU-2022923Reactome: -MMU-2024101 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinExtracellular SideApical Cell MembraneCell ProjectionLamellipodium MembraneCell SurfaceLocalized At The Apical Plasma Membrane It Relocalizes To The Lamellipodia Of Astrocytoma Upon Phosphorylation By PrkcaLocalizes To The Retraction FibersA Fraction May Undergo Cell Surface Proteolysis And SecretionLocalizes To The Plasma Membrane Of Oligodendrocytes |
| Alternative Antibody Pair Names | Chondroitin Sulfate Proteoglycan 4 antibody pairChondroitin Sulfate Proteoglycan Ng2 antibody pairProteoglycan An2 antibody pairCspg4 antibody pairAn2 antibody pairKiaa4232 antibody pairNg2 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Mouse NG2 (NG2) Antibody Pair (STJAP00801)
This antibody pair is designed to detect mouse NG2 (UniProt Q8VHY0) and has confirmed reactivity in mouse. It is intended for use in sandwich ELISA-based research applications.
Both antibodies are rabbit monoclonal IgGs supplied unconjugated at 1 mg/mL in 0.01M PBS, pH 7.2. The clone IDs are R53-2Z-1 and R14-7S-4, and both are affinity purified.
This pair is validated for ELISA. Both antibodies are unconjugated, so end users will need to label the detection antibody or use an appropriate secondary detection reagent as part of their assay setup.
Both antibodies were raised in rabbit against a recombinant protein immunogen corresponding to mouse NG2. No specific immunogen region is listed in the product details.
Both components should be stored at 4°C for short-term use. For long-term storage, aliquot and store at -20°C. Repeated freeze/thaw cycles should be avoided to maintain antibody integrity.
Optimal working dilutions for both the capture and detection antibodies are to be determined by the end user through titration. No predefined dilution ranges are specified for this pair.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.