Mouse IL-5 (Interleukin 5) Sandwich ELISA Kit (STJE0019975)

Name: Mouse IL-5 (Interleukin 5) Sandwich ELISA Kit (STJE0019975)
SKU: STJE0019975
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

STJE0019975

Current Stock:

🚚 Free UK Delivery on orders over £150

Quantity:

Processing The item has been added

Enquire For Bulk Order

✓ 1-Year Performance Guarantee | SDS & Datasheet Included | Expert Tech Support | Covered by St John's Laboratory Guarantee

Short Description	This IL-5 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Mouse
Sensitivity	2.21 pg/mL
Detection Limit	3.91-250 pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Gene Symbol	Il5
Gene ID	16191
Uniprot ID	IL5_MOUSE
Specificity	This kit recognizes Mouse IL-5 in samples. No significant cross-reactivity or interference between Mouse IL-5 and analogues was observed.
Sample Type	serum, plasma and other biological fluids

Tissue Specificity	Expressed in lymphoid cells, including spleen, thymus, lymph nodes and peripheral blood mononuclear cells.
Function	Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils. Also acts on activated and resting B-cells to induce immunoglobulin production, growth, and differentiation. Mechanistically, exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB. Binding to the receptor leads to activation of various kinases including LYN, SYK and JAK2 and thereby propagates signals through the RAS-MAPK and JAK-STAT5 pathways respectively.
Protein Name	Interleukin-5 Il-5 B-Cell Growth Factor Ii Bcgf-Ii Cytotoxic T-Lymphocyte Inducer Eosinophil Differentiation Factor T-Cell Replacing Factor Trf
Database Links	Reactome: R-MMU-512988 Reactome: -MMU-5673001 Reactome: -MMU-912526
Cellular Localisation	Secreted
Alternative ELISA Names	Interleukin-5 ELISA kit Il-5 ELISA kit B-Cell Growth Factor Ii ELISA kit Bcgf-Ii ELISA kit Cytotoxic T-Lymphocyte Inducer ELISA kit Eosinophil Differentiation Factor ELISA kit T-Cell Replacing Factor ELISA kit Trf ELISA kit Il5 ELISA kit Il-5 ELISA kit
Specificity	This kit recognizes Mouse IL-5 in samples. No significant cross-reactivity or interference between Mouse IL-5 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (50×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (50×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	12.71	39.49	84.60	13.91	36.07	78.94
Standard deviation	0.65	1.62	2.83	0.72	1.89	2.60
CV (%)	5.12	4.11	3.34	5.15	5.24	3.29