Mouse Hepc (Hepcidin) CLIA Kit for Quantitative Detection
SPECIFICATIONS
General Information
| Short Description | This mouse Hepc kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Applications | CLIA |
| Reactivity | Mouse |
| Sensitivity | 18.75pg/mL |
| Detection Limit | 31.25~2000pg/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Chemiluminescence |
Target Information
| Gene Symbol | Hamp |
| Gene ID | 84506 |
| Uniprot ID | HEPC_MOUSE |
| Specificity | This kit recognizes Mouse Hepc in samples. No significant cross-reactivity or interference between Mouse Hepc and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Tissue Specificity | Highly expressed in the liver and to a much lesser extent in the heart. Secreted in blood. |
| Function | Liver-produced hormone that constitutes the main circulating regulator of iron absorption and distribution across tissues. Acts by promoting endocytosis and degradation of SLC40A1, leading to the retention of iron in iron-exporting cells and decreased flow of iron into plasma. Controls the major flows of iron into plasma: absorption of dietary iron in the intestine, recycling of iron by macrophages, which phagocytose old erythrocytes and other cells, and mobilization of stored iron from hepatocytes. |
| Protein Name | Hepcidin |
| Database Links | |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Hepcidin CLIA kitHamp CLIA kitHamp1 CLIA kitHepc CLIA kitHepc1 CLIA kit |
| Specificity | This kit recognizes Mouse Hepc in samples. No significant cross-reactivity or interference between Mouse Hepc and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 90-101 | 95 |
| EDTA plasma(n=8) | 86-99 | 92 |
| Cell culture media(n=8) | 83-96 | 90 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 110.04 | 302.39 | 988.50 | 100.07 | 298.43 | 904.19 |
| Standard deviation | 10.27 | 26.19 | 79.48 | 10.59 | 34.32 | 81.20 |
| CV (%) | 9.33 | 8.66 | 8.04 | 10.58 | 11.50 | 8.98 |