| Applications: | ELISA |
| Reactivity: | Mouse |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Sensitivity: | 0.117ng/mL |
| Detection Limit: | 0.312-20ng/mL |
| Short Description: | This GLS Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse tissue homogenates, cell lysates or other biological fluids.. |
| Storage Instruction: | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time: | 4.5 hrs |
| Gene Symbol: | Gls |
| Gene ID: | 14660 |
| Uniprot ID: | GLSK_MOUSE |
| Sample Type: | tissue homogenates, cell lysates or other biological fluids. |
| Tissue Specificity | |
| Post Translational Modifications | Synthesized as a 74-kDa cytosolic precursor which is proteolytically processed by the mitochondrial-processing peptidase (MPP) via a 72-kDa intermediate to yield the mature mitochondrial 68- and 65-kDa subunits. |
| Function | Catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Plays a role in maintaining acid-base homeostasis. Regulates the levels of the neurotransmitter glutamate, the main excitatory neurotransmitter in the brain. |
| Protein Name | Glutaminase Kidney Isoform - Mitochondrial Gls Cleaved Into - Glutaminase Kidney Isoform - Mitochondrial 68 Kda Chain - Glutaminase Kidney Isoform - Mitochondrial 65 Kda Chain |
| Database Links | Reactome: R-MMU-210500 Reactome: -MMU-5628897 Reactome: -MMU-8964539 |
| Cellular Localisation | Isoform 1: Mitochondrion Cytoplasm Cytosol The 74-Kda Cytosolic Precursor Is Translocated Into The Mitochondria And Processed Via A 72-Kda Intermediate To Yield The Mature 68- And 65-Kda Subunits Isoform 2: Mitochondrion Glutaminase Kidney Isoform Mitochondrial 68 Kda Chain: Mitochondrion Matrix Produced By The Proteolytic Processing Of The 74-Kda Cytosolic Precursor Mitochondrial 65 Kda Chain: Mitochondrion Matrix |
| Alternative ELISA Names | Glutaminase Kidney Isoform - Mitochondrial ELISA kit Gls Cleaved Into - Glutaminase Kidney Isoform - Mitochondrial 68 Kda Chain - Glutaminase Kidney Isoform - Mitochondrial 65 Kda Chain ELISA kit Gls ELISA kit Gls1 ELISA kit Kiaa0838 ELISA kit |
| output |
Information sourced from Uniprot.org
