| Applications: |
CLIA |
| Reactivity: |
Mouse |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
37.5pg/mL |
| Detection Limit: |
62.5~4000pg/mL |
| Short Description: |
This mouse F8 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
F8 |
| Gene ID: |
14069 |
| Uniprot ID: |
FA8_MOUSE |
| Specificity: |
This kit recognizes Mouse F8 in samples. No significant cross-reactivity or interference between Mouse F8 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Found in most tissues. |
| Post Translational Modifications | The binding of vWF and activation depend on the sulfation of Tyr-1669. Proteolytically cleaved by cathepsin CTSG to produce a partially activated form. |
| Function | Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa. |
| Protein Name | Coagulation Factor ViiiProcoagulant Component |
| Database Links | Reactome: R-MMU-114608Reactome: -MMU-140837Reactome: -MMU-140875Reactome: -MMU-163841Reactome: -MMU-204005Reactome: -MMU-5694530 |
| Cellular Localisation | SecretedExtracellular Space |
| Alternative CLIA Names | Coagulation Factor Viii CLIA kitProcoagulant Component CLIA kitF8 CLIA kitCf8 CLIA kitF8c CLIA kit |
| Specificity | This kit recognizes Mouse F8 in samples. No significant cross-reactivity or interference between Mouse F8 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 95-108 | 102 |
| EDTA plasma(n=8) | 93-104 | 98 |
| Cell culture media(n=8) | 94-108 | 101 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 217.64 | 590.35 | 1347.16 | 212.80 | 532.03 | 1268.09 |
| Standard deviation | 22.90 | 51.83 | 146.84 | 27.49 | 39.96 | 108.55 |
| CV (%) | 10.52 | 8.78 | 10.90 | 12.92 | 7.51 | 8.56 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
