| Host: |
HEK293 cells |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: |
Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: |
<1 EU/ug of the protein by LAL method. |
| Immunoreactivity: |
Recombinant Mouse EGFR/HER1 Protein captured on CM5 Chip via anti-his antibody can bind Mouse EGF, hFc Tag with an affinity constant of 9.96 nM as determined in SPR assay (Biacore T200). |
| Gene Symbol: |
Egfr |
| Gene ID: |
13649 |
| Uniprot ID: |
EGFR_MOUSE |
| Immunogen Region: |
Leu25-Ser647 |
| Immunogen: |
Recombinant Mouse EGFR/HER1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Leu25-Ser647) of Mouse EGFR/HER1 (Accession #NP_997538.1) fused with a His tag at the C-terminus. |
| Immunogen Sequence: |
PSGPISTINPCPPCKECHKC PAPNLEGGPSVFIFPPNIKD VLMISLTPKVTCVVVDVSED DPDVQISWFVNNVEVHTAQT QTHREDYNSTIRVVSTLPIQ HQDWMSGKEFKCKVNNKDLP SPIERTISKIKGLVRAPQVY ILPPPAEQLSRKDVSLTCLV VGFNPGDISVEWTSNGHTEE NYKDTAPVLDSDGSYFIYSK LNMKTSKWEKTDSFSCNVRH EGLKNYYLKKTISRSPGK |
| Post Translational Modifications | Monoubiquitinated and polyubiquitinated upon EGF stimulation.which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occurs. Deubiquitinated by OTUD7B, preventing degradation. Ubiquitinated by RNF115 and RNF126. Ubiquitinated by ZNRF1 or CBL at different lysines in response to EGF stimulation.leading to recruitment of the ESCRT machinery and subsequent degradation in the lysosomes. Deubiquitinated by UCHL1 leading to the inhibition of its degradation. Phosphorylated on Tyr residues in response to EGF. Phosphorylation at Ser-697 is partial and occurs only if Thr-695 is phosphorylated. Phosphorylation at Thr-680 and Thr-695 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1199 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2. Palmitoylated on Cys residues by ZDHHC20. Palmitoylation inhibits internalization after ligand binding, and increases the persistence of tyrosine-phosphorylated EGFR at the cell membrane. Palmitoylation increases the amplitude and duration of EGFR signaling. Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197. |
| Function | Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling. Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin. Positively regulates cell migration via interaction with CCDC88A/GIV which retains EGFR at the cell membrane following ligand stimulation, promoting EGFR signaling which triggers cell migration. Plays a role in enhancing learning and memory performance. Plays a role in mammalian pain signaling (long-lasting hypersensitivity). |
| Protein Name | Epidermal Growth Factor Receptor |
| Database Links | Reactome: R-MMU-1227986Reactome: -MMU-1236394Reactome: -MMU-1250196Reactome: -MMU-1257604Reactome: -MMU-177929Reactome: -MMU-179812Reactome: -MMU-180292Reactome: -MMU-180336Reactome: -MMU-182971Reactome: -MMU-1963642Reactome: -MMU-212718Reactome: -MMU-2179392Reactome: -MMU-445144Reactome: -MMU-5673001Reactome: -MMU-6785631Reactome: -MMU-6811558Reactome: -MMU-8847993Reactome: -MMU-8856825Reactome: -MMU-8856828Reactome: -MMU-8857538Reactome: -MMU-8863795Reactome: -MMU-9009391Reactome: -MMU-9013507 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinEndoplasmic Reticulum MembraneGolgi Apparatus MembraneNucleus MembraneEndosomeEndosome MembraneNucleusIn Response To EgfTranslocated From The Cell Membrane To The Nucleus Via Golgi And ErEndocytosed Upon Activation By LigandColocalized With Gper1 In The Nucleus Of Estrogen Agonist-Induced Cancer-Associated Fibroblasts (Caf) |
| Alternative Protein Names | Epidermal Growth Factor Receptor proteinEgfr protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
