Mouse E-Cad (Cadherin-1) Antibody Pair (STJAP00534)
SPECIFICATIONS
HostRabbit/Goat
ConjugationUnconjugated/Biotin
ImmunogenCapture immunogen: Recombinant Mouse E-Cad protien.Detection Immunogen: Recombinant Mouse E-Cad protien.
General Information
| Short Description | This E-Cad antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rabbit/Goat |
| Reactivity | Mouse |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Antigen Affinity.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | Cdh1 |
| Gene ID | 12550 |
| Uniprot ID | CADH1_MOUSE |
| Immunogen | Capture immunogen: Recombinant Mouse E-Cad protien.Detection Immunogen: Recombinant Mouse E-Cad protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | During apoptosis or with calcium influx, cleaved by a membrane-bound metalloproteinase (ADAM10), PS1/gamma-secretase and caspase-3. Processing by the metalloproteinase, induced by calcium influx, causes disruption of cell-cell adhesion and the subsequent release of beta-catenin into the cytoplasm. The residual membrane-tethered cleavage product is rapidly degraded via an intracellular proteolytic pathway. Cleavage by caspase-3 releases the cytoplasmic tail resulting in disintegration of the actin microfilament system. The gamma-secretase-mediated cleavage promotes disassembly of adherens junctions. During development of the cochlear organ of Corti, cleavage by ADAM10 at adherens junctions promotes pillar cell separation. O-glycosylated. O-manosylated by TMTC1, TMTC2, TMTC3 or TMTC4. Ser-287 and Thr-511 are O-manosylated by TMTC2 or TMTC4 but not TMTC1 or TMTC3. N-glycosylation at Asn-639 is essential for expression, folding and trafficking. Addition of bisecting N-acetylglucosamine by MGAT3 modulates its cell membrane location. Ubiquitinated by a SCF complex containing SKP2, which requires prior phosphorylation by CK1/CSNK1A1. Ubiquitinated by CBLL1/HAKAI, requires prior phosphorylation at Tyr-756. |
| Function | Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells.cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Promotes organization of radial actin fiber structure and cellular response to contractile forces, via its interaction with AMOTL2 which facilitates anchoring of radial actin fibers to CDH1 junction complexes at the cell membrane. Plays a role in the early stages of desmosome cell-cell junction formation via facilitating the recruitment of DSG2 and DSP to desmosome plaques. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production. (Microbial infection) Does not function as a receptor for L.monocytogenes internalin A (InlA).mutating a single surface-exposed residue confers receptor activity to this protein and promotes uptake of the bacteria. |
| Protein Name | Cadherin-1Arc-1Epithelial CadherinE-CadherinUvomorulinCd Antigen Cd324 Cleaved Into - E-Cad/Ctf1 - E-Cad/Ctf2 - E-Cad/Ctf3 |
| Database Links | Reactome: R-MMU-1474228Reactome: -MMU-216083Reactome: -MMU-351906Reactome: -MMU-418990Reactome: -MMU-5626467 |
| Cellular Localisation | Cell JunctionAdherens JunctionCell MembraneSingle-Pass Type I Membrane ProteinEndosomeGolgi ApparatusTrans-Golgi NetworkCytoplasmDesmosomeColocalizes With Dlgap5 At Sites Of Cell-Cell Contact In Intestinal Epithelial CellsAnchored To Actin Microfilaments Through Association With Alpha-Beta- And Gamma-CateninSequential Proteolysis Induced By Apoptosis Or Calcium InfluxResults In Translocation From Sites Of Cell-Cell Contact To The CytoplasmColocalizes With Rab11a Endosomes During Its Transport From The Golgi Apparatus To The Plasma MembraneRecruited To Desmosomes At The Initial Assembly Phase And Also Accumulates Progressively At Mature Desmosome Cell-Cell JunctionsLocalizes To Cell-Cell Contacts As Keratinocyte Differentiation Progresses |
| Alternative Antibody Pair Names | Cadherin-1 antibody pairArc-1 antibody pairEpithelial Cadherin antibody pairE-Cadherin antibody pairUvomorulin antibody pairCd Antigen Cd324 Cleaved Into - E-Cad/Ctf1 - E-Cad/Ctf2 - E-Cad/Ctf3 antibody pairCdh1 antibody pair |
Information sourced from Uniprot.org