Mouse CD36 protein (Recombinant) (STJP001878)

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STJP001878

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Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Mouse CD36-protein was developed from hek293. For use in research applications.
Conjugation: Unconjugated
Formulation: Lyophilised from 0.2 Mu m filtered PBS solution, pH7.2, 5% Trehalose.
Storage Instruction: Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA
Immunoreactivity: The activity was tested by the ability of immobilized recombinant Mouse CD36 to bind Human RSPO1 with a linear range of 6-800 ng/ml NA
Gene Symbol: Cd36
Gene ID: 12491
Uniprot ID: CD36_MOUSE
Immunogen Region: ECD
Immunogen: Optimized DNA sequence encoding extracellular domain of Mouse CD36 including a C-terminal His tag was expressed in HEK293 cells. NA
Tissue Specificity Expressed in the apical side of lingual taste bud cells of the circumvallate papillae. Highly expressed in the intestine on the luminal surface of enterocytes. In small intestines expression levels follow a steep decreasing gradient from proximal to distal segments. Expressed in macrophages. Cell surface expression detected in lung alveolar macrophages, dendritic macrophages and lung macrophages (at protein level).
Post Translational Modifications Palmitoylated by ZDHHC5. Palmitoylation is required for proper localization at the plasma membrane. Ubiquitinated at Lys-469 and Lys-472. Ubiquitination is induced by fatty acids such as oleic acid and leads to degradation by the proteasome. Ubiquitination and degradation are inhibited by insulin which blocks the effect of fatty acids.
Function Multifunctional glycoprotein that acts as receptor for a broad range of ligands. Ligands can be of proteinaceous nature like thrombospondin, fibronectin, collagen or amyloid-beta as well as of lipidic nature such as oxidized low-density lipoprotein (oxLDL), anionic phospholipids, long-chain fatty acids and bacterial diacylated lipopeptides. They are generally multivalent and can therefore engage multiple receptors simultaneously, the resulting formation of CD36 clusters initiates signal transduction and internalization of receptor-ligand complexes. The dependency on coreceptor signaling is strongly ligand specific. Cellular responses to these ligands are involved in angiogenesis, inflammatory response, fatty acid metabolism, taste and dietary fat processing in the intestine (Probable). Binds long-chain fatty acids and facilitates their transport into cells, thus participating in muscle lipid utilization, adipose energy storage, and gut fat absorption. Mechanistically, binding of fatty acids activates downstream kinase LYN, which phosphorylates the palmitoyltransferase ZDHHC5 and inactivates it, resulting in the subsequent depalmitoylation of CD36 and caveolar endocytosis. In the small intestine, plays a role in proximal absorption of dietary fatty acid and cholesterol for optimal chylomicron formation, possibly through the activation of MAPK1/3 (ERK1/2) signaling pathway. Involved in oral fat perception and preferences. Detection into the tongue of long-chain fatty acids leads to a rapid and sustained rise in flux and protein content of pancreatobiliary secretions. In taste receptor cells, mediates the induction of an increase in intracellular calcium levels by long-chain fatty acids, leading to the activation of the gustatory neurons in the nucleus of the solitary tract. Important factor in both ventromedial hypothalamus neuronal sensing of long-chain fatty acid and the regulation of energy and glucose homeostasis. Receptor for thrombospondins, THBS1 and THBS2, mediating their antiangiogenic effects. Involved in inducing apoptosis in podocytes in response to elevated free fatty acids, acting together with THBS1. As a coreceptor for TLR4:TLR6 heterodimer, promotes inflammation in monocytes/macrophages. Upon ligand binding, such as oxLDL or amyloid-beta 42, interacts with the heterodimer TLR4:TLR6, the complex is internalized and triggers inflammatory response, leading to NF-kappa-B-dependent production of CXCL1, CXCL2 and CCL9 cytokines, via MYD88 signaling pathway, and CCL5 cytokine, via TICAM1 signaling pathway, as well as IL1B secretion, through the priming and activation of the NLRP3 inflammasome. Selective and nonredundant sensor of microbial diacylated lipopeptide that signal via TLR2:TLR6 heterodimer, this cluster triggers signaling from the cell surface, leading to the NF-kappa-B-dependent production of TNF, via MYD88 signaling pathway and subsequently is targeted to the Golgi in a lipid-raft dependent pathway. (Microbial infection) Acts as an accessory receptor for M.tuberculosis lipoprotein LprA, in conjunction with coreceptors TLR2 and TLR1.the lipoprotein acts as an agonist to modulate antigen presenting cell functions in response to the pathogen. Directly mediates cytoadherence of Plasmodium falciparum parasitized erythrocytes and the internalization of particles independently of TLR signaling. Mediates uptake of E.coli and S.aureus but has no effect on uptake of M.fortuitum.
Protein Name Platelet Glycoprotein 4
Glycoprotein Iiib
Gpiiib
Pas Iv
Pas-4
Platelet Glycoprotein Iv
Gpiv
Cd Antigen Cd36
Database Links Reactome: R-MMU-114608
Reactome: -MMU-1236973
Reactome: -MMU-3000471
Reactome: -MMU-434313
Reactome: -MMU-5686938
Reactome: -MMU-6798695
Cellular Localisation Cell Membrane
Multi-Pass Membrane Protein
Apical Cell Membrane
Membrane Raft
Golgi Apparatus
Upon Ligand-Binding
Internalized Through Dynamin-Dependent Endocytosis
Alternative Protein Names Platelet Glycoprotein 4 protein
Glycoprotein Iiib protein
Gpiiib protein
Pas Iv protein
Pas-4 protein
Platelet Glycoprotein Iv protein
Gpiv protein
Cd Antigen Cd36 protein
Cd36 protein

Information sourced from Uniprot.org

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