Mouse Ccl11 (Eotaxin) Sandwich ELISA Kit (STJE0005342)

Name: Mouse Ccl11 (Eotaxin) Sandwich ELISA Kit (STJE0005342)
SKU: STJE0005342
Availability: InStock

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SPECIFICATIONS

STJE0005342

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Short Description	This Ccl11 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Mouse
Sensitivity	37.5ng/mL
Detection Limit	62.5~4000ng/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Colormetric

Gene Symbol	Ccl11
Gene ID	20292
Uniprot ID	CCL11_MOUSE
Specificity	This kit recognizes Mouse ECF/CCL11 in samples. No significant cross-reactivity or interference between Mouse ECF/CCL11 and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Function	In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils (a prominent feature of allergic inflammatory reactions), but not lymphocytes, macrophages or neutrophils. Binds to CCR3.
Protein Name	Eotaxin C-C Motif Chemokine 11 Eosinophil Chemotactic Protein Small-Inducible Cytokine A11
Database Links	Reactome: R-MMU-380108 Reactome: -MMU-418594
Cellular Localisation	Secreted
Alternative ELISA Names	Eotaxin ELISA kit C-C Motif Chemokine 11 ELISA kit Eosinophil Chemotactic Protein ELISA kit Small-Inducible Cytokine A11 ELISA kit Ccl11 ELISA kit Scya11 ELISA kit
Specificity	This kit recognizes Mouse ECF/CCL11 in samples. No significant cross-reactivity or interference between Mouse ECF/CCL11 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	167.16	438.75	1630.13	171.09	463.75	1723.54
Standard deviation	8.68	21.54	66.51	9.96	21.15	71.53
CV (%)	5.19	4.91	4.08	5.82	4.56	4.15