Mouse ALPL (ALPL) Antibody Pair (STJAP00958)
General Information
| Short Description | This ALPL antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Mouse |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL/0.5-4ug/mL |
| Dilution Range | 1:1000-1:10000 |
| Storage Instruction | Store at-20Β°C for the long term, and avoid freeze-thaw cycles. |
| Detection | Colorimetric method;ELISA;Sandwich |
Target Information
| Gene Symbol | Alpl |
| Gene ID | 11647 |
| Uniprot ID | PPBT_MOUSE |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | N-glycosylated. |
| Function | Alkaline phosphatase that metabolizes various phosphate compounds and plays a key role in skeletal mineralization and adaptive thermogenesis. Has broad substrate specificity and can hydrolyze a considerable variety of compounds: however, only a few substrates, such as diphosphate (inorganic pyrophosphate.PPi), pyridoxal 5'-phosphate (PLP) and N-phosphocreatine are natural substrates. Plays an essential role in skeletal and dental mineralization via its ability to hydrolyze extracellular diphosphate, a potent mineralization inhibitor, to phosphate: it thereby promotes hydroxyapatite crystal formation and increases inorganic phosphate concentration. Acts in a non-redundant manner with PHOSPHO1 in skeletal mineralization: while PHOSPHO1 mediates the initiation of hydroxyapatite crystallization in the matrix vesicles (MVs), ALPL/TNAP catalyzes the spread of hydroxyapatite crystallization in the extracellular matrix. Also promotes dephosphorylation of osteopontin (SSP1), an inhibitor of hydroxyapatite crystallization in its phosphorylated state.it is however unclear whether ALPL/TNAP mediates SSP1 dephosphorylation via a direct or indirect manner. Catalyzes dephosphorylation of PLP to pyridoxal (PL), the transportable form of vitamin B6, in order to provide a sufficient amount of PLP in the brain, an essential cofactor for enzymes catalyzing the synthesis of diverse neurotransmitters. Additionally, also able to mediate ATP degradation in a stepwise manner to adenosine, thereby regulating the availability of ligands for purinergic receptors. Also capable of dephosphorylating microbial products, such as lipopolysaccharides (LPS) as well as other phosphorylated small-molecules, such as poly-inosine:cytosine (poly I:C). Acts as a key regulator of adaptive thermogenesis as part of the futile creatine cycle: localizes to the mitochondria of thermogenic fat cells and acts by mediating hydrolysis of N-phosphocreatine to initiate a futile cycle of creatine dephosphorylation and phosphorylation. During the futile creatine cycle, creatine and N-phosphocreatine are in a futile cycle, which dissipates the high energy charge of N-phosphocreatine as heat without performing any mechanical or chemical work. |
| Protein Name | Alkaline Phosphatase - Tissue-Nonspecific IsozymeAp-TnapTnapTnsalpAlkaline Phosphatase 2Alkaline Phosphatase Liver/Bone/Kidney IsozymePhosphoamidasePhosphocreatine Phosphatase |
| Database Links | Reactome: R-MMU-163125 |
| Cellular Localisation | Cell MembraneLipid-AnchorGpi-AnchorExtracellular Vesicle MembraneMitochondrion MembraneMitochondrion Intermembrane SpaceLocalizes To Special Class Of Extracellular VesiclesNamed Matrix Vesicles (Mvs)Which Are Released By Osteogenic CellsLocalizes To The Mitochondria Of Thermogenic Fat Cells: Tethered To Mitochondrial Membranes Via A Gpi-Anchor And Probably Resides In The Mitochondrion Intermembrane Space |
| Alternative Antibody Pair Names | Alkaline Phosphatase - Tissue-Nonspecific Isozyme antibody pairAp-Tnap antibody pairTnap antibody pairTnsalp antibody pairAlkaline Phosphatase 2 antibody pairAlkaline Phosphatase Liver/Bone/Kidney Isozyme antibody pairPhosphoamidase antibody pairPhosphocreatine Phosphatase antibody pairAlpl antibody pairAkp-2 antibody pairAkp2 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Mouse ALPL (ALPL) Antibody Pair (STJAP00958)
This antibody pair is designed to detect mouse ALPL (alkaline phosphatase, tissue-nonspecific isozyme), corresponding to UniProt accession P09242. Reactivity has been confirmed for mouse samples only.
The pair consists of two rabbit-raised antibodies: the capture antibody is unconjugated at a concentration of 0.5 mg/mL, and the detection antibody is biotin-conjugated at a concentration of 0.5 to 4 ug/mL.
This antibody pair is validated for use in sandwich ELISA with a colorimetric detection method. It is not validated for other assay formats such as Western blot or immunohistochemistry.
This antibody pair has been tested with serum, plasma, and other biological fluids from mouse origin.
A working dilution range of 1:1000 to 1:10000 is recommended. Optimal dilutions should be determined empirically for each specific experimental setup.
Both components should be stored at -20 degrees Celsius for long-term storage. Repeated freeze-thaw cycles should be avoided to maintain reagent integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.