• Human VTN (Vitronectin) CLIA Kit (STJC0000519)

Human VTN (Vitronectin) CLIA Kit (STJC0000519)

SKU:
STJC0000519

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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.56ng/mL
Detection Limit: 0.94~60ng/mL
Short Description: This human VTN kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: VTN
Gene ID: 7448
Uniprot ID: VTNC_HUMAN
Specificity: This kit recognizes Human VTN in samples. No significant cross-reactivity or interference between Human VTN and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Expressed in the retina pigment epithelium (at protein level). Expressed in plasma (at protein level). Expressed in serum (at protein level).
Post Translational Modifications Sulfated on tyrosine residues. N- and O-glycosylated. Phosphorylation on Thr-69 and Thr-76 favors cell adhesion and spreading. It has been suggested that the active SMB domain may be permitted considerable disulfide bond heterogeneity or variability, thus two alternate disulfide patterns based on 3D structures are described with 1 disulfide bond conserved in both. Phosphorylation sites are present in the extracellular medium.
Function Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway. Somatomedin-B is a growth hormone-dependent serum factor with protease-inhibiting activity.
Protein Name Vitronectin
Vn
S-Protein
Serum-Spreading Factor
V75 Cleaved Into - Vitronectin V65 Subunit - Vitronectin V10 Subunit - Somatomedin-B
Database Links Reactome: R-HSA-2129379
Reactome: R-HSA-216083
Reactome: R-HSA-3000170
Reactome: R-HSA-3000178
Reactome: R-HSA-977606
Cellular Localisation Secreted
Extracellular Space
Parasitophorous Vacuole
(Microbial Infection) In P
Falciparum-Infected Red Blood Cells
Vtn Internalization Is Detected At The Early Trophozoite Stage
Colocalizes With Sera5 At The Schizont Stage And With Sera5 P47 At The Merozoite Surface
Alternative CLIA Names Vitronectin CLIA kit
Vn CLIA kit
S-Protein CLIA kit
Serum-Spreading Factor CLIA kit
V75 Cleaved Into - Vitronectin V65 Subunit - Vitronectin V10 Subunit - Somatomedin-B CLIA kit
VTN CLIA kit
Specificity This kit recognizes Human VTN in samples. No significant cross-reactivity or interference between Human VTN and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 100-116 108
EDTA plasma(n=8) 92-105 97
Cell culture media(n=8) 93-104 99

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 3.28 6.12 29.70 3.57 6.62 28.39
Standard deviation 0.34 0.46 2.71 0.35 0.55 2.49
CV (%) 10.37 7.52 9.12 9.80 8.31 8.77
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance