Human Vimentin (Vimentin) Antibody Pair (STJAP00838)
General Information
| Short Description | This Vimentin antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R51-7Q-8/R63-2Z-8 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | VIM |
| Gene ID | 7431 |
| Uniprot ID | VIME_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Tissue Specificity | Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. |
| Post Translational Modifications | Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin. One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33. Phosphorylated on tyrosine residues by SRMS. O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status. S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. |
| Function | Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Plays a role in cell directional movement, orientation, cell sheet organization and Golgi complex polarization at the cell migration front. Protects SCRIB from proteasomal degradation and facilitates its localization to intermediate filaments in a cell contact-mediated manner. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. |
| Protein Name | Vimentin |
| Database Links | Reactome: R-HSA-264870Reactome: R-HSA-390522Reactome: R-HSA-6785807Reactome: R-HSA-9013422Reactome: R-HSA-9613829Reactome: R-HSA-9615710Reactome: R-HSA-9646399 |
| Cellular Localisation | CytoplasmCytoskeletonNucleus MatrixCell Membrane |
| Alternative Antibody Pair Names | Vimentin antibody pairVIM antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human Vimentin (Vimentin) Antibody Pair (STJAP00838)
This antibody pair is designed to detect human Vimentin (UniProt P08670). Reactivity has been confirmed for human samples only.
This antibody pair has been validated for use in ELISA. It is intended for research use only and is not validated for diagnostic or therapeutic applications.
Both antibodies are rabbit monoclonal IgGs supplied unconjugated at 1 mg/mL in 0.01M PBS, pH 7.2. They are affinity purified and carry clone IDs R51-7Q-8 and R63-2Z-8 respectively.
Both components should be stored at 4°C for short-term use. For long-term storage, aliquot and store at -20°C, and avoid repeated freeze/thaw cycles.
Working concentrations for both the capture and detection antibodies are to be determined by the end user. Titration experiments are recommended to establish optimal conditions for each specific assay setup.
Both antibodies were raised in rabbit against a recombinant human Vimentin protein. No specific immunogen region is defined in the product specifications.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.