This Vascular Endothelial Growth Factor A Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma, cell culture supernates.
Applications
ELISA
Reactivity
Human
Sensitivity
7.8 pg/mL
Detection Limit
312-2000 pg/mL
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Conjugation
Biotin
Storage Instruction
Unopened kits can be stored at 2-8°C for 1 year, and opened products must be used within 1 month.
Higher expression in pituitary tumors than the pituitary gland. Isoform VEGF189: Widely expressed. Isoform VEGF165: Widely expressed. Isoform VEGF121: Widely expressed. Isoform VEGF206: Not widely expressed. Isoform VEGF145: Not widely expressed.
Post Translational Modifications
Vascular endothelial growth factor A, long form: Produced by use of an alternative upstream CUG codon and post-translationally processed into the N-terminal N-VEGF form and the C-terminal secreted VEGFA form.
Function
N-VEGF: Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A. Involved in protecting cells from hypoxia-mediated cell death. VEGFA: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development. Also binds the DEAR/FBXW7-AS1 receptor. Isoform VEGF165B: Binds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
Protein Name
Vascular Endothelial Growth Factor A - Long FormL-VegfVascular Permeability FactorVpf Cleaved Into - N-Vegf - Vegfa
N-Vegf: CytoplasmNucleusCytoplasmic In Normoxic Conditions And Localizes To The Nucleus Under Hypoxic ConditionsVegfa: SecretedIsoform L-Vegf189: Endoplasmic ReticulumGolgi ApparatusSecretedExtracellular SpaceExtracellular MatrixIsoform Vegf121: SecretedIsoform Vegf165: SecretedIsoform Vegf189: SecretedCell-Associated After Secretion And Is Bound Avidly By Heparin And The Extracellular MatrixAlthough It May Be Released As A Soluble Form By HeparinHeparinase Or Plasmin
Alternative ELISA Names
Vascular Endothelial Growth Factor A - Long Form ELISA kitL-Vegf ELISA kitVascular Permeability Factor ELISA kitVpf Cleaved Into - N-Vegf - Vegfa ELISA kitVEGFA ELISA kitVEGF ELISA kit
