| Short Description : | Recombinant-Human Ubc13/UBE2N-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications: | SDS-PAGE |
| Host: | E.coli |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Concentration: | 0.5 mg/mL |
| Formulation: | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 20% Glycerol, 0.1M NaCl, 1mM DTT |
| Storage Instruction: | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | UBE2N |
| Gene ID: | 7334 |
| Uniprot ID: | UBE2N_HUMAN |
| Accession Number: | NP_003339 |
| Immunogen: | Human |
| Immunogen Region: | 1-152aa |
| Immunogen Sequence: |
| Post Translational Modifications | Conjugation to ISG15 impairs formation of the thioester bond with ubiquitin but not interaction with UBE2V2. |
| Function | The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD. Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes. Together with RNF135 and UB2V1, catalyzes the viral RNA-dependent 'Lys-63'-linked polyubiquitination of RIGI to activate the downstream signaling pathway that leads to interferon beta production. UBE2V1-UBE2N together with TRAF3IP2 E3 ubiquitin ligase mediate 'Lys-63'-linked polyubiquitination of TRAF6, a component of IL17A-mediated signaling pathway. |
| Protein Name | Ubiquitin-Conjugating Enzyme E2 NBendless-Like Ubiquitin-Conjugating EnzymeE2 Ubiquitin-Conjugating Enzyme NUbc13Ubch13Ubiquitin Carrier Protein NUbiquitin-Protein Ligase N |
| Database Links | Reactome: R-HSA-1169408Reactome: R-HSA-168638Reactome: R-HSA-168927Reactome: R-HSA-202424Reactome: R-HSA-2871837Reactome: R-HSA-445989Reactome: R-HSA-450302Reactome: R-HSA-450321Reactome: R-HSA-5205685Reactome: R-HSA-5607764Reactome: R-HSA-5693565Reactome: R-HSA-5693571Reactome: R-HSA-5693607Reactome: R-HSA-5696395Reactome: R-HSA-69473Reactome: R-HSA-8866654Reactome: R-HSA-9020702Reactome: R-HSA-937039Reactome: R-HSA-937041Reactome: R-HSA-9646399Reactome: R-HSA-9705671Reactome: R-HSA-975110Reactome: R-HSA-975144Reactome: R-HSA-983168 |
| Cellular Localisation | NucleusCytoplasm |
| Alternative Protein Names | Ubiquitin-Conjugating Enzyme E2 N proteinBendless-Like Ubiquitin-Conjugating Enzyme proteinE2 Ubiquitin-Conjugating Enzyme N proteinUbc13 proteinUbch13 proteinUbiquitin Carrier Protein N proteinUbiquitin-Protein Ligase N proteinUBE2N proteinBLU protein |
Information sourced from Uniprot.org
![Anti-Ube2N/Ubc13 antibody [SM5128] (STJA0005128)](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/180181/403618/STJA0005128_1__27833.1738950626.png?c=1 "Anti-Ube2N/Ubc13 antibody [SM5128] (STJA0005128)")
