• Human TXNRD1 (Thioredoxin reductase 1, cytoplasmic) CLIA Kit (STJC0000765)

Human TXNRD1 (Thioredoxin reductase 1, cytoplasmic) CLIA Kit (STJC0000765)

SKU:
STJC0000765

Shipping:
Free Shipping
Current Stock:
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This human TXNRD1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: TXNRD1
Gene ID: 7296
Uniprot ID: TRXR1_HUMAN
Specificity: This kit recognizes Human TrxR1 in samples. No significant cross-reactivity or interference between Human TrxR1 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Isoform 1: Expressed predominantly in Leydig cells (at protein level). Also expressed in ovary, spleen, heart, liver, kidney and pancreas and in a number of cancer cell lines. Isoform 4: Widely expressed with highest levels in kidney, testis, uterus, ovary, prostate, placenta and fetal liver.
Post Translational Modifications Isoform 5: The N-terminus is blocked. ISGylated.
Function Reduces disulfideprotein thioredoxin (Trx) to its dithiol-containing form. Homodimeric flavoprotein involved in the regulation of cellular redox reactions, growth and differentiation. Contains a selenocysteine residue at the C-terminal active site that is essential for catalysis (Probable). Also has reductase activity on hydrogen peroxide (H2O2). Isoform 1: Induces actin and tubulin polymerization, leading to formation of cell membrane protrusions. Isoform 4: Enhances the transcriptional activity of estrogen receptors ESR1 and ESR2. Isoform 5: Enhances the transcriptional activity of the estrogen receptor ESR2 only. Mediates cell death induced by a combination of interferon-beta and retinoic acid.
Protein Name Thioredoxin Reductase 1 - Cytoplasmic
Tr
Gene Associated With Retinoic And Interferon-Induced Mortality 12 Protein
Grim-12
Gene Associated With Retinoic And Ifn-Induced Mortality 12 Protein
Km-102-Derived Reductase-Like Factor
Peroxidase Txnrd1
Thioredoxin Reductase Tr1
Database Links Reactome: R-HSA-1989781
Reactome: R-HSA-2408550
Reactome: R-HSA-3299685
Reactome: R-HSA-499943
Reactome: R-HSA-5263617
Reactome: R-HSA-5336415
Reactome: R-HSA-5628897
Reactome: R-HSA-9818027
Cellular Localisation Isoform 1: Cytoplasm
Isoform 4: Cytoplasm
Nucleus
Isoform 5: Cytoplasm
Alternative CLIA Names Thioredoxin Reductase 1 - Cytoplasmic CLIA kit
Tr CLIA kit
Gene Associated With Retinoic And Interferon-Induced Mortality 12 Protein CLIA kit
Grim-12 CLIA kit
Gene Associated With Retinoic And Ifn-Induced Mortality 12 Protein CLIA kit
Km-102-Derived Reductase-Like Factor CLIA kit
Peroxidase Txnrd1 CLIA kit
Thioredoxin Reductase Tr1 CLIA kit
TXNRD1 CLIA kit
GRIM12 CLIA kit
KDRF CLIA kit
Specificity This kit recognizes Human TrxR1 in samples. No significant cross-reactivity or interference between Human TrxR1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-104 96
EDTA plasma(n=8) 89-102 96
Cell culture media(n=8) 93-106 100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 94.97 300.95 929.28 99.80 279.57 841.33
Standard deviation 11.90 35.78 77.32 9.01 21.47 85.56
CV (%) 12.53 11.89 8.32 9.03 7.68 10.17
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance