This human TXN kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.
Applications
CLIA
Reactivity
Human
Sensitivity
37.5pg/mL
Detection Limit
62.5~4000pg/mL
Note
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Product Properties
Storage Instruction
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
This kit recognizes Human Trx in samples. No significant cross-reactivity or interference between Human Trx and analogues was observed.
Sample Type
Serum, plasma and other biological fluids
Additional Info
Post Translational Modifications
In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins. In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation.
Function
Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity. ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).
Protein Name
ThioredoxinTrxAtl-Derived FactorAdfSurface-Associated Sulphydryl ProteinSaspAllergen Hom S Trx
NucleusCytoplasmSecretedTranslocates From The Cytoplasm Into The Nucleus After Phorbol 12-Myristate 13-Acetate Induction (Pma)Predominantly In The Cytoplasm In Non Irradiated CellsRadiation Induces Translocation Of Trx From The Cytoplasm To The NucleusSecreted By A Leaderless Secretory Pathway
Alternative CLIA Names
Thioredoxin CLIA kitTrx CLIA kitAtl-Derived Factor CLIA kitAdf CLIA kitSurface-Associated Sulphydryl Protein CLIA kitSasp CLIA kitAllergen Hom S Trx CLIA kitTXN CLIA kitTRDX CLIA kitTRX CLIA kitTRX1 CLIA kit
Specificity
This kit recognizes Human Trx in samples. No significant cross-reactivity or interference between Human Trx and analogues was observed.
