Human TSP-1 (Thrombospondin-1) Antibody Pair (STJAP00207)
General Information
| Short Description | This TSP-1 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Mouse/Goat |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Polyclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | THBS1 |
| Gene ID | 7057 |
| Uniprot ID | TSP1_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human TSP-1 protein.Detection Immunogen: Recombinant Human TSP-1 protein. |
Additional Info
| Function | Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Multifunctional, involved in inflammation, angiogenesis, wound healing, reactive oxygen species (ROS) signaling, nitrous oxide (NO) signaling, apoptosis, senescence, aging, cellular self-renewal, stemness, and cardiovascular and metabolic homeostasis. Negatively modulates dendritic cell activation and cytokine release, as part of an autocrine feedback loop, contributing to the resolution of inflammation and immune homeostasis. Ligand for receptor CD47. Modulates nitrous oxide (NO) signaling via CD47, hence playing a role as a pressor agent, supporting blood pressure. Plays a role in endothelial cell senescence, acting via CD47, by increasing the abundance and activation of NADPH oxidase NOX1, and so generating excess ROS. Inhibits stem cell self-renewal, acting via CD47 signaling, probably by regulation of the stem cell transcription factors POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4. Negatively modulates wound healing, acting via CD47. Ligand for receptor CD36. Involved in inducing apoptosis in podocytes in response to elevated free fatty acids, acting via CD36. Plays a role in suppressing angiogenesis, acting, depending on context, via CD36 or CD47. Promotes cellular senescence in a TP53-CDKN1A-RB1 signaling-dependent manner. Ligand for immunoglobulin-like cell surface receptor SIRPA. Involved in ROS signaling in non-phagocytic cells, stimulating NADPH oxidase-derived ROS production, acting via interaction with SIRPA. Plays a role in metabolic dysfunction in diet-induced obesity, perhaps acting by exacerbating adipose inflammatory activity.its effects may be mediated, at least in part, through enhanced adipocyte proliferation. Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors. May be involved in age-related conditions, including metabolic dysregulation, during normal aging. |
| Protein Name | Thrombospondin-1Glycoprotein G |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-186797Reactome: R-HSA-216083Reactome: R-HSA-3000170Reactome: R-HSA-5083635Reactome: R-HSA-5173214Reactome: R-HSA-8936459 |
| Cellular Localisation | SecretedCell SurfaceExtracellular SpaceExtracellular MatrixEndoplasmic ReticulumSarcoplasmic ReticulumSecreted By Thrombin-Activated Platelets And Binds To The Cell Surface In The Presence Of Extracellular Ca(2+)Incorporated Into The Extracellular Matrix (Ecm) Of FibroblastsThe C-Terminal Region In Trimeric Form Is Required For Retention In The EcmAlso Detected In The Endoplasmic Reticulum And Sarcoplasmic Reticulum Where It Plays A Role In The Er Stress Response |
| Alternative Antibody Pair Names | Thrombospondin-1 antibody pairGlycoprotein G antibody pairTHBS1 antibody pairTSP antibody pairTSP1 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human TSP-1 (Thrombospondin-1) Antibody Pair (STJAP00207)
This antibody pair is designed to detect human Thrombospondin-1 (TSP-1), corresponding to UniProt entry P07996. Reactivity is confirmed for human samples only.
The capture antibody is an unconjugated mouse monoclonal antibody, and the detection antibody is a biotin-conjugated goat polyclonal antibody. Both are supplied at 0.5 mg/mL in PBS containing 0.04% Proclin 300 and 50% glycerol at pH 7.4.
This pair has been validated for use in ELISA. No other assay formats are listed in the product specifications.
Both the capture and detection antibodies were raised against recombinant human TSP-1 protein. The capture antibody was purified using Protein A or G, while the detection antibody was purified by antigen affinity.
Both components should be stored at -20°C for long-term storage. Repeated freeze-thaw cycles should be avoided to preserve antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.