Human TSG101 protein (Recombinant) (His-Tag) (STJP016671)

SPECIFICATIONS
HostE.coli
ImmunogenHuman
STJP016671
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General Information

Short DescriptionRecombinant-Human TSG101-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications.
ApplicationsSDS-PAGE
HostE.coli
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

Concentration0.5 mg/mL
FormulationLiquid in 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% Glycerol
Storage InstructionFor short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolTSG101
Gene ID7251
Uniprot IDTS101_HUMAN
Accession NumberNP_006283
ImmunogenHuman
Immunogen Region1-145aa
Immunogen SequenceMRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMAVS ESQLKKMVSK YKYRDLTVRE TVNVITLYKD LKPVLDSYVF NDGSSRELMN LTGTIPVPYR GNTYNIPICL WLLDTYPYNP PICFVKPTSS MTIKTGKHVD ANGKIYLPYL HEWKHPQSDL LGLIQVMIVV FGDEPPVFSR P

Additional Info

Tissue Specificity Heart, brain, placenta, lung, liver, skeletal, kidney and pancreas.
Post Translational Modifications Monoubiquitinated at multiple sites by LRSAM1 and by MGRN1. Ubiquitination inactivates it, possibly by regulating its shuttling between an active membrane-bound protein and an inactive soluble form. Ubiquitination by MGRN1 requires the presence of UBE2D1.
Function Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis.the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-ST-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Required for the exosomal release of SDCBP, CD63 and syndecan. It may also play a role in the extracellular release of microvesicles that differ from the exosomes.
Protein Name Tumor Susceptibility Gene 101 Protein
Escrt-I Complex Subunit Tsg101
Database Links Reactome: R-HSA-162588
Reactome: R-HSA-174490
Reactome: R-HSA-917729
Reactome: R-HSA-9610379
Reactome: R-HSA-9615710
Cellular Localisation Cytoplasm
Early Endosome Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Late Endosome Membrane
Cytoskeleton
Microtubule Organizing Center
Centrosome
Midbody
Midbody Ring
Nucleus
Mainly Cytoplasmic
Membrane-Associated When Active And Soluble When Inactive
Nuclear Localization Is Cell Cycle-Dependent
Interaction With Cep55 Is Required For Localization To The Midbody During Cytokinesis
Alternative Protein Names Tumor Susceptibility Gene 101 Protein protein
Escrt-I Complex Subunit Tsg101 protein
TSG101 protein

Information sourced from Uniprot.org

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