Human TRPV1 protein (Recombinant) (N-His) (STJP005720)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human TRPV1-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | TRPV1 |
| Gene ID | 7442 |
| Uniprot ID | TRPV1_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Met1-Glu153 |
Additional Info
| Post Translational Modifications | Phosphorylation by PKA reverses capsaicin-induced dephosphorylation at multiple sites, probably including Ser-117 as a major phosphorylation site. Phosphorylation by CAMKII seems to regulate binding to vanilloids. Phosphorylated and modulated by PRKCE, PRKCM and probably PRKCZ. Dephosphorylation by calcineurin seems to lead to receptor desensitization and phosphorylation by CAMKII recovers activity. |
| Function | Non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. Involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Activated by vanilloids, like capsaicin, and temperatures higher than 42 degrees Celsius. Upon activation, exhibits a time- and Ca(2+)-dependent outward rectification, followed by a long-lasting refractory state. Mild extracellular acidic pH (6.5) potentiates channel activation by noxious heat and vanilloids, whereas acidic conditions (pH <6) directly activate the channel. Can be activated by endogenous compounds, including 12-hydroperoxytetraenoic acid and bradykinin. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accumbens by affecting AMPA receptors endocytosis. |
| Protein Name | Transient Receptor Potential Cation Channel Subfamily V Member 1Trpv1Capsaicin ReceptorOsm-9-Like Trp Channel 1Otrpc1Vanilloid Receptor 1 |
| Database Links | Reactome: R-HSA-3295583 |
| Cellular Localisation | Postsynaptic Cell MembraneMulti-Pass Membrane ProteinCell ProjectionDendritic Spine MembraneCell MembraneMostlyBut Not Exclusively Expressed In Postsynaptic Dendritic Spines |
| Alternative Protein Names | Transient Receptor Potential Cation Channel Subfamily V Member 1 proteinTrpv1 proteinCapsaicin Receptor proteinOsm-9-Like Trp Channel 1 proteinOtrpc1 proteinVanilloid Receptor 1 proteinTRPV1 proteinVR1 protein |
Information sourced from Uniprot.org