• Biotinylated Human TRAIL R1 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
  • The purity of Biotinylated Human TRAIL R1 is greater than 95% as determined by SEC-HPLC.
  • Immobilized Human TRAIL, No Tag at 5 Mu g/ml (100 Mu l/well) on the plate. Dose response curve for Biotinylated Human TRAIL R1, His Tag with the EC50 of 0. 31 Mu g/ml determined by ELISA.
  • Biotinylated Human TRAIL R1, His-Avi Tag immobilized on CM5 Chip can bind Human TRAIL, No Tag with an affinity constant of 0. 315 nM as determined in SPR assay (Biacore T200).

Human TRAIL R1/DR4/TNFRSF10A protein (Recombinant) (C-His-Avi) {Biotin} (STJP001687)

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STJP001687

£618.50 - £1,909.50
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Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human TRAIL R1/DR4/TNFRSF10A-C-His-Avi protein was developed from hek293 and has a target region of C-His-Avi. For use in research applications.
Conjugation: Biotin
Formulation: Lyophilised from 0.2 µm filtered PBS solution pH7.4.
Dilution Range: Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM
Storage Instruction: The lyophilized protein is stable for at least 1 year from date of receipt at-20°C.
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg).
Gene Symbol: TNFRSF10A
Gene ID: 8797
Uniprot ID: TR10A_HUMAN
Immunogen Region: Pro34-Asn239
Immunogen: DNA sequence encoding Human TRAIL R1/DR4/TNFRSF10A including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Post Translational Modifications Palmitoylated. Palmitoylation of TNFRSF10A is required for its association with lipid rafts, oligomerization and function in TRAIL-induced cell death. Palmitoylated by ZDHHC3 (Probable).
Function Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B.
Protein Name Tumor Necrosis Factor Receptor Superfamily Member 10a
Death Receptor 4
Tnf-Related Apoptosis-Inducing Ligand Receptor 1
Trail Receptor 1
Trail-R1
Cd Antigen Cd261
Database Links Reactome: R-HSA-140534
Reactome: R-HSA-202733
Reactome: R-HSA-3371378
Reactome: R-HSA-5213460
Reactome: R-HSA-5218900
Reactome: R-HSA-6803211
Reactome: R-HSA-69416
Reactome: R-HSA-75158
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Membrane Raft
Cytoplasm
Cytosol
Palmitoylation Is Required For Association With Membranes
Alternative Protein Names Tumor Necrosis Factor Receptor Superfamily Member 10a protein
Death Receptor 4 protein
Tnf-Related Apoptosis-Inducing Ligand Receptor 1 protein
Trail Receptor 1 protein
Trail-R1 protein
Cd Antigen Cd261 protein
TNFRSF10A protein
APO2 protein
DR4 protein
TRAILR1 protein

Information sourced from Uniprot.org

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