Human tPA (Tissue-Type Plasminogen Activator) Antibody Pair (STJAP00253)
General Information
| Short Description | This tPA antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Polyclonal/Polyclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Protein A or G. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | PLAT |
| Gene ID | 5327 |
| Uniprot ID | TPA_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human tPA protein.Detection Immunogen: Recombinant Human tPA protein. |
Additional Info
| Post Translational Modifications | The single chain, almost fully active enzyme, can be further processed into a two-chain fully active form by a cleavage after Arg-310 catalyzed by plasmin, tissue kallikrein or factor Xa. Differential cell-specific N-linked glycosylation gives rise to two glycoforms, type I (glycosylated at Asn-219) and type II (not glycosylated at Asn-219). The single chain type I glycoform is less readily converted into the two-chain form by plasmin, and the two-chain type I glycoform has a lower activity than the two-chain type II glycoform in the presence of fibrin. N-glycosylation of Asn-152.the bound oligomannosidic glycan is involved in the interaction with the mannose receptor. Characterization of O-linked glycan was studied in Bowes melanoma cell line. |
| Function | Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. During oocyte activation, plays a role in cortical granule reaction in the zona reaction, which contributes to the block to polyspermy. |
| Protein Name | Tissue-Type Plasminogen ActivatorT-PaT-Plasminogen ActivatorTpaAlteplaseReteplase Cleaved Into - Tissue-Type Plasminogen Activator Chain A - Tissue-Type Plasminogen Activator Chain B |
| Database Links | Reactome: R-HSA-186797Reactome: R-HSA-75205 |
| Cellular Localisation | SecretedExtracellular Space |
| Alternative Antibody Pair Names | Tissue-Type Plasminogen Activator antibody pairT-Pa antibody pairT-Plasminogen Activator antibody pairTpa antibody pairAlteplase antibody pairReteplase Cleaved Into - Tissue-Type Plasminogen Activator Chain A - Tissue-Type Plasminogen Activator Chain B antibody pairPLAT antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human tPA (Tissue-Type Plasminogen Activator) Antibody Pair (STJAP00253)
This antibody pair detects human Tissue-Type Plasminogen Activator (tPA), corresponding to UniProt entry P00750. Reactivity is confirmed for human samples only.
Both antibodies are rabbit polyclonal antibodies supplied at 0.5 mg/mL. The capture antibody is unconjugated, and the detection antibody is biotinylated. Both are formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.4.
This antibody pair is validated for use in ELISA. No other assay formats are listed in the product specifications.
Both antibodies were raised against recombinant human tPA protein. The same immunogen type was used for both the capture and detection antibody.
Both components should be stored at -20 degrees Celsius for long-term storage. Freeze-thaw cycles should be avoided to maintain reagent integrity.
Both antibodies were purified using Protein A or Protein G affinity chromatography.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.