Human SPAM1 (PH-20) protein (Recombinant) (STJP000531)

Conjugation:	Unconjugated
Formulation:	Lyophilised from 0.2 Mu m filtered PBS solution, pH7.2, 5% Trehalose.
Dilution Range:	Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers.
Storage Instruction:	Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA
Endotoxin:	Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA

Gene Symbol:	SPAM1
Gene ID:	6677
Uniprot ID:	HYALP_HUMAN
Immunogen Region:	ECD
Immunogen:	Optimized DNA sequence encoding extracellular domain of human SPAM1 (PH20) (LEU36-TYR482 ) including a C-terminal His tag was expressed in HEK293 cells. NA

Tissue Specificity	Testis.
Post Translational Modifications	N-glycosylated.
Function	Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.
Protein Name	Hyaluronidase Ph-20 Hyal-Ph20 Hyaluronoglucosaminidase Ph-20 Sperm Adhesion Molecule 1 Sperm Surface Protein Ph-20
Database Links	Reactome: R-HSA-2534343
Cellular Localisation	Cell Membrane Lipid-Anchor Gpi-Anchor
Alternative Protein Names	Hyaluronidase Ph-20 protein Hyal-Ph20 protein Hyaluronoglucosaminidase Ph-20 protein Sperm Adhesion Molecule 1 protein Sperm Surface Protein Ph-20 protein SPAM1 protein HYAL3 protein PH20 protein

Information sourced from Uniprot.org

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