Human SMO protein (Recombinant) (N-His) (STJP005916)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human SMO-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | SMO |
| Gene ID | 6608 |
| Uniprot ID | SMO_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Ser32-Tyr233 |
Additional Info
| Function | G protein-coupled receptor which associates with the patched protein (PTCH) to transduce hedgehog protein signaling. Binding of sonic hedgehog (SHH) to its receptor patched prevents inhibition of smoothened (SMO) by patched. When active, SMO binds to and sequesters protein kinase A catalytic subunit PRKACA at the cell membrane, preventing PRKACA-mediated phosphorylation of GLI transcription factors which releases the GLI proteins from PRKACA-mediated inhibition and allows for transcriptional activation of hedgehog pathway target genes. Required for the accumulation of KIF7, GLI2 and GLI3 in the cilia. Interacts with DLG5 at the ciliary base to induce the accumulation of KIF7 and GLI2 at the ciliary tip for GLI2 activation. |
| Protein Name | Protein SmoothenedProtein Gx |
| Database Links | Reactome: R-HSA-373080Reactome: R-HSA-5610787Reactome: R-HSA-5620922Reactome: R-HSA-5632684Reactome: R-HSA-5635838 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinCell ProjectionCiliumCilium Localization Is Promoted By Shh And Is Required For Activity |
| Alternative Protein Names | Protein Smoothened proteinProtein Gx proteinSMO proteinSMOH protein |
Information sourced from Uniprot.org