Human SIRT4 protein (Recombinant) (N-His) (STJP006293)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human SIRT4-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | SIRT4 |
| Gene ID | 23409 |
| Uniprot ID | SIR4_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Ser28-Cys314 |
Additional Info
| Function | Acts as a NAD-dependent protein lipoamidase, biotinylase, deacetylase and ADP-ribosyl transferase. Catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications. Inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner. Catalyzes the transfer of ADP-ribosyl groups onto target proteins, including mitochondrial GLUD1, inhibiting GLUD1 enzyme activity. Acts as a negative regulator of mitochondrial glutamine metabolism by mediating mono ADP-ribosylation of GLUD1: expressed in response to DNA damage and negatively regulates anaplerosis by inhibiting GLUD1, leading to block metabolism of glutamine into tricarboxylic acid cycle and promoting cell cycle arrest. In response to mTORC1 signal, SIRT4 expression is repressed, promoting anaplerosis and cell proliferation. Acts as a tumor suppressor. Also acts as a NAD-dependent protein deacetylase: mediates deacetylation of 'Lys-471' of MLYCD, inhibiting its activity, thereby acting as a regulator of lipid homeostasis. Does not seem to deacetylate PC. Controls fatty acid oxidation by inhibiting PPARA transcriptional activation. Impairs SIRT1-PPARA interaction probably through the regulation of NAD(+) levels. Down-regulates insulin secretion. |
| Protein Name | Nad-Dependent Protein Lipoamidase Sirtuin-4 - MitochondrialNad-Dependent Adp-Ribosyltransferase Sirtuin-4Nad-Dependent Protein Biotinylase Sirtuin-4Nad-Dependent Protein Deacetylase Sirtuin-4Regulatory Protein Sir2 Homolog 4Sir2-Like Protein 4 |
| Database Links | Reactome: R-HSA-204174Reactome: R-HSA-2151201 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Protein Names | Nad-Dependent Protein Lipoamidase Sirtuin-4 - Mitochondrial proteinNad-Dependent Adp-Ribosyltransferase Sirtuin-4 proteinNad-Dependent Protein Biotinylase Sirtuin-4 proteinNad-Dependent Protein Deacetylase Sirtuin-4 proteinRegulatory Protein Sir2 Homolog 4 proteinSir2-Like Protein 4 proteinSIRT4 proteinSIR2L4 protein |
Information sourced from Uniprot.org