Human Siglec-4/MAG/GMA protein (Recombinant) (C-hFc&His) (STJP019813)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human Siglec-4/MAG/GMA Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly20-Pro516) of Human Siglec-4/MAG/GMA (Accession #NP_002352.1) fused with hFc tag and His tag at the C-terminus
General Information
| Short Description | Recombinant-Human Siglec-4/MAG/GMA-C-hFc&His protein was developed in hek293 cells using the region Gly20-Pro516. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | MAG |
| Gene ID | 4099 |
| Uniprot ID | MAG_HUMAN |
| Immunogen | Recombinant Human Siglec-4/MAG/GMA Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly20-Pro516) of Human Siglec-4/MAG/GMA (Accession #NP_002352.1) fused with hFc tag and His tag at the C-terminus |
| Immunogen Region | Gly20-Pro516 |
| Immunogen Sequence | GHWGAWMPSSISAFEGTCVS IPCRFDFPDELRPAVVHGVW YFNSPYPKNYPPVVFKSRTQ VVHESFQGRSRLLGDLGLRN CTLLLSNVSPELGGKYYFRG DLGGYNQYTFSEHSVLDIVN TPNIVVPPEVVAGTEVEVSC MVPDNCPELRPELSWLGHEG LGEPAVLGRLREDEGTWVQV SLLHFVPTREANGHRLGCQA SFPNTTLQFEGYASMDVKYP PVIVEMNSSVEAIEGSHVS |
Additional Info
| Tissue Specificity | Both isoform 1 and isoform 2 are detected in myelinated structures in the central and peripheral nervous system, in periaxonal myelin and at Schmidt-Lanterman incisures. Detected in optic nerve, in oligodendroglia and in periaxonal myelin sheaths. Detected in compact myelin (at protein level). Both isoform 1 and isoform 2 are detected in the central and peripheral nervous system. |
| Post Translational Modifications | N-glycosylated. Phosphorylated on tyrosine residues. Ubiquitinated, leading to proteasomal degradation. |
| Function | Adhesion molecule that mediates interactions between myelinating cells and neurons by binding to neuronal sialic acid-containing gangliosides and to the glycoproteins RTN4R and RTN4RL2. Not required for initial myelination, but seems to play a role in the maintenance of normal axon myelination. Protects motoneurons against apoptosis, also after injury.protection against apoptosis is probably mediated via interaction with neuronal RTN4R and RTN4RL2. Required to prevent degeneration of myelinated axons in adults.this probably depends on binding to gangliosides on the axon cell membrane. Negative regulator of neurite outgrowth.in dorsal root ganglion neurons the inhibition is mediated primarily via binding to neuronal RTN4R or RTN4RL2 and to a lesser degree via binding to neuronal gangliosides. In cerebellar granule cells the inhibition is mediated primarily via binding to neuronal gangliosides. In sensory neurons, inhibition of neurite extension depends only partially on RTN4R, RTN4RL2 and gangliosides. Inhibits axon longitudinal growth. Inhibits axon outgrowth by binding to RTN4R. Preferentially binds to alpha-2,3-linked sialic acid. Binds ganglioside Gt1b. |
| Protein Name | Myelin-Associated GlycoproteinSiglec-4a |
| Database Links | Reactome: R-HSA-193634Reactome: R-HSA-210991Reactome: R-HSA-9619665 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinMembrane Raft |
| Alternative Protein Names | Myelin-Associated GlycoproteinSiglec-4a proteinMAG proteinGMA protein |
Information sourced from Uniprot.org