| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of 50mM Tris, 150mM NaCl, pH 7.5. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | < 0.1 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: | Measured by its ability to inhibit Recombinant Human Coagulation Factor II/Thrombin cleavage of a fluorogenic peptide substrate Boc-VPR-AMC.The IC50 value is <0.19 nM. |
| Gene Symbol: | SERPIND1 |
| Gene ID: | 3053 |
| Uniprot ID: | HEP2_HUMAN |
| Immunogen: | Recombinant Human Serpin D1/Heparin cofactor 2 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly20-Ser499) of human Serpin D1/Heparin Cofactor II (Accession #NP_000176.2.) fused with a 6×His tag |
| Immunogen Sequence: | GSKGPLDQLEKGGETAQSAD PQWEQLNNKNLSMPLLPADF HKENTVTNDWIPEGEEDDDY LDLEKIFSEDDDYIDIVDSL SVSPTDSDVSAGNILQLFHG KSRIQRLNIVNAKFAFNLYR VLKDQVNTFDNIFIAPVGIS TAMGMISLGLKGETHEQVHS ILHFKDFVNASSKYEITTIH NLFRKLTHRLFRRNFGYTLR SVNDLYIQKQFPILLDFKTK VREYYFAEAQIADFSDPAF |
| Post Translational Modifications | Phosphorylated by FAM20C in the extracellular medium. |
| Function | Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III). Also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner. Peptides at the N-terminal of HC-II have chemotactic activity for both monocytes and neutrophils. Isoform 2: Shows negligible inhibition, in vitro, of thrombin and tPA and no inhibition of factor Xa, in vitro. |
| Protein Name | Heparin Cofactor 2Heparin Cofactor IiHc-IiProtease Inhibitor Leuserpin-2Hls2Serpin D1 |
| Database Links | Reactome: R-HSA-140837Reactome: R-HSA-140875Reactome: R-HSA-381426Reactome: R-HSA-8957275 |
| Alternative Protein Names | Heparin Cofactor 2 proteinHeparin Cofactor Ii proteinHc-Ii proteinProtease Inhibitor Leuserpin-2 proteinHls2 proteinSerpin D1 proteinSERPIND1 proteinHCF2 protein |
Information sourced from Uniprot.org
