Human Serpin D1/Heparin cofactor 2 protein (Recombinant) (C-His) (STJP001220)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human Serpin D1/Heparin cofactor 2 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly20-Ser499) of human Serpin D1/Heparin Cofactor II (Accession #NP_000176.2.) fused with a 6×His tag
General Information
| Short Description | Recombinant-Human Serpin D1/Heparin cofactor 2-C-His protein was developed in hek293 cells using the region Gly20-Ser499. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of 50mM Tris, 150mM NaCl, pH 7.5. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its ability to inhibit Recombinant Human Coagulation Factor II/Thrombin cleavage of a fluorogenic peptide substrate Boc-VPR-AMC.The IC50 value is <0.19 nM. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | SERPIND1 |
| Gene ID | 3053 |
| Uniprot ID | HEP2_HUMAN |
| Immunogen | Recombinant Human Serpin D1/Heparin cofactor 2 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gly20-Ser499) of human Serpin D1/Heparin Cofactor II (Accession #NP_000176.2.) fused with a 6×His tag |
| Immunogen Region | Gly20-Ser499 |
| Immunogen Sequence | GSKGPLDQLEKGGETAQSAD PQWEQLNNKNLSMPLLPADF HKENTVTNDWIPEGEEDDDY LDLEKIFSEDDDYIDIVDSL SVSPTDSDVSAGNILQLFHG KSRIQRLNIVNAKFAFNLYR VLKDQVNTFDNIFIAPVGIS TAMGMISLGLKGETHEQVHS ILHFKDFVNASSKYEITTIH NLFRKLTHRLFRRNFGYTLR SVNDLYIQKQFPILLDFKTK VREYYFAEAQIADFSDPAF |
Additional Info
| Post Translational Modifications | Phosphorylated by FAM20C in the extracellular medium. |
| Function | Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT-III). Also inhibits chymotrypsin, but in a glycosaminoglycan-independent manner. Peptides at the N-terminal of HC-II have chemotactic activity for both monocytes and neutrophils. Isoform 2: Shows negligible inhibition, in vitro, of thrombin and tPA and no inhibition of factor Xa, in vitro. |
| Protein Name | Heparin Cofactor 2Heparin Cofactor IiHc-IiProtease Inhibitor Leuserpin-2Hls2Serpin D1 |
| Database Links | Reactome: R-HSA-140837Reactome: R-HSA-140875Reactome: R-HSA-381426Reactome: R-HSA-8957275 |
| Cellular Localisation | |
| Alternative Protein Names | Heparin Cofactor 2 proteinHeparin Cofactor Ii proteinHc-Ii proteinProtease Inhibitor Leuserpin-2 proteinHls2 proteinSerpin D1 proteinSERPIND1 proteinHCF2 protein |
Information sourced from Uniprot.org