| Host: | HEK293 cells |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Formulation: | Lyophilised from a 0.22 Mu m filtered solution of 20mM HEPES, 150mM NaCl, pH 8.0. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method: | < 0.1 EU/Mu g of the protein by LAL method. |
| Immunoreactivity: | Measured by its ability to inhibit chymotrypsin cleavage of a fluorogenic peptide substrate MCA-RPKPVE-Nval-WRK (Dnp)-NH2. The IC50 value is <11.5 nM. |
| Gene Symbol: | SERPINA3 |
| Gene ID: | 12 |
| Uniprot ID: | AACT_HUMAN |
| Immunogen: | Recombinant Human Serpin A3/Alpha-1-antichymotrypsin Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Asn26-Ala423) of human Serpin A3/AACT (Accession #NP_001076.2) fused with a 6×His tag at the C-termin |
| Immunogen Sequence: | NSPLDEENLTQENQDRGTHV DLGLASANVDFAFSLYKQLV LKAPDKNVIFSPLSISTALA FLSLGAHNTTLTEILKGLKF NLTETSEAEIHQSFQHLLRT LNQSSDELQLSMGNAMFVKE QLSLLDRFTEDAKRLYGSEA FATDFQDSAAAKKLINDYVK NGTRGKITDLIKDLDSQTMM VLVNYIFFKAKWEMPFDPQD THQSRFYLSKKKWVMVPMMS LHHLTIPYFRDEELSCTVV |
| Tissue Specificity | Plasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains. |
| Post Translational Modifications | N- and O-glycosylated. |
| Function | Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2. |
| Protein Name | Alpha-1-AntichymotrypsinActCell Growth-Inhibiting Gene 24/25 ProteinSerpin A3 Cleaved Into - Alpha-1-Antichymotrypsin His-Pro-Less |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-6798695 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Alpha-1-Antichymotrypsin proteinAct proteinCell Growth-Inhibiting Gene 24/25 Protein proteinSerpin A3 Cleaved Into - Alpha-1-Antichymotrypsin His-Pro-Less proteinSERPINA3 proteinAACT proteinGIG24 proteinGIG25 protein |
Information sourced from Uniprot.org