Human RPN1 protein (Recombinant) (N-His) (STJP003683)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human RPN1-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | RPN1 |
| Gene ID | 6184 |
| Uniprot ID | RPN1_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Ala24-Ser179 |
Additional Info
| Post Translational Modifications | Ubiquitinated by the ECS(ASB11) complex. Ubiquitinated by RNF128, leading to degradation in a proteasome/lysosome-dependent manner. Ufmylated by UFL1 in response to endoplasmic reticulum stress, promoting reticulophagy of endoplasmic reticulum sheets. |
| Function | Subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol-pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. |
| Protein Name | Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase Subunit 1Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase 67 Kda SubunitRibophorin IRpn-IRibophorin-1 |
| Database Links | Reactome: R-HSA-1799339Reactome: R-HSA-446203Reactome: R-HSA-9694548 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Type I Membrane ProteinMelanosomeIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv |
| Alternative Protein Names | Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase Subunit 1 proteinDolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase 67 Kda Subunit proteinRibophorin I proteinRpn-I proteinRibophorin-1 proteinRPN1 protein |
Information sourced from Uniprot.org