Human RhoG protein (Recombinant) (His-Tag) (STJP017705)

SPECIFICATIONS
HostE.coli
ImmunogenHuman
STJP017705
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General Information

Short DescriptionRecombinant-Human RhoG-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications.
ApplicationsSDS-PAGE
HostE.coli
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

Concentration0.25 mg/mL
FormulationLiquid in 20mM Tris-HCl buffer (pH 8.0) 20% Glycerol, 50mM Imidazole, 0.5M NaCl
Storage InstructionFor short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolRHOG
Gene ID391
Uniprot IDRHOG_HUMAN
Accession NumberNP_001656
ImmunogenHuman
Immunogen Region1-188aa
Immunogen SequenceMQS IKCVVVGDGA VGKTCLLICY TTNAFPKEYI PTVFDNYSAQ SAVDGRTVNL NLWDTAGQEE YDRLRTLSYP QTNVFVICFS IASPPSYENV RHKWHPEVCH HCPDVPILLV GTKKDLRAQP DTLRRLKEQG QAPITPQQGQ ALAKQIHAVR YLECSALQQD GVKEVFAEAV RAVLNPTPIK RGRSC

Additional Info

Post Translational Modifications (Microbial infection) Glucosylated at Thr-35 by C.difficile toxin TcdB. (Microbial infection) Glycosylated (O-GlcNAcylated) at Thr-35 by C.novyi toxin TcdA. O-GlcNAcylation completely prevents the recognition of the downstream effector, blocking the GTPases in their inactive form, leading to actin cytoskeleton disruption.
Function Plays a role in immunological synaptic F-actin density and architecture organization. Regulates actin reorganization in lymphocytes, possibly through the modulation of Rac1 activity. Required for the formation of membrane ruffles during macropinocytosis. Plays a role in cell migration and is required for the formation of cup-like structures during trans-endothelial migration of leukocytes. Binds phospholipids in an activation-dependent manner.thereby acting as an anchor for other proteins to the plasma membrane (PM). Plays a role in exocytosis of cytotoxic granules (CG) by lymphocytes/Component of the exocytosis machinery in natural killer (NK) and CD8+ T cells. Promotes the docking of cytotoxic granules (CG) to the plasma membrane through the interaction with UNC13D. Involved in the cytotoxic activity of lymphocytes/primary CD8+ T cells. (Microbial infection) In case of Salmonella enterica infection, activated by SopB and ARHGEF26/SGEF, which induces cytoskeleton rearrangements and promotes bacterial entry.
Protein Name Rho-Related Gtp-Binding Protein Rhog
Database Links Reactome: R-HSA-114604
Reactome: R-HSA-1257604
Reactome: R-HSA-2219530
Reactome: R-HSA-5625970
Reactome: R-HSA-6798695
Reactome: R-HSA-6811558
Reactome: R-HSA-9013408
Cellular Localisation Cell Membrane
Lipid-Anchor
Cytoplasmic Side
Alternative Protein Names Rho-Related Gtp-Binding Protein Rhog protein
RHOG protein
ARHG protein

Information sourced from Uniprot.org

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