Human RANGAP1 protein (Recombinant) (N-His) (STJP016105)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human RANGAP1-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
| Endotoxin | Please contact us for further information. |
Target Information
| Gene Symbol | RANGAP1 |
| Gene ID | 5905 |
| Uniprot ID | RAGP1_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Gly21-Asp360 |
Additional Info
| Post Translational Modifications | Phosphorylation occurs before nuclear envelope breakdown and continues throughout mitosis. Phosphorylated by the M-phase kinase cyclin B/Cdk1, in vitro. Differential timimg of dephosphorylation occurs during phases of mitosis. The phosphorylated form remains associated with RANBP2/NUP358 and the SUMO E2-conjugating enzyme, UBE2I, on nuclear pore complex (NPC) diassembly and during mitosis. Sumoylated. Sumoylation is necessary for targeting to the nuclear envelope (NE), and for association with mitotic spindles and kinetochores during mitosis. Also required for interaction with RANBP2 and is mediated by UBE2I. Desumoylated by HINT1. |
| Function | GTPase activator for RAN. Converts cytoplasmic GTP-bound RAN to GDP-bound RAN, which is essential for RAN-mediated nuclear import and export. Mediates dissociation of cargo from nuclear export complexes containing XPO1, RAN and RANBP2 after nuclear export. |
| Protein Name | Ran Gtpase-Activating Protein 1Rangap1 |
| Database Links | Reactome: R-HSA-141444Reactome: R-HSA-165054Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-4615885Reactome: R-HSA-5663220Reactome: R-HSA-68877Reactome: R-HSA-9615933Reactome: R-HSA-9648025Reactome: R-HSA-9793242 |
| Cellular Localisation | CytoplasmNucleusNucleoplasmNucleus EnvelopeChromosomeCentromereKinetochoreCytoskeletonSpindleCytoplasmic During InterphaseDetected At The Nuclear Envelope During InterphaseTargeted To The Nuclear Pores After SumoylationDuring MitosisAssociates With Mitotic SpindlesBut Is Essentially Not Detected At The Spindle PolesAssociation With Kinetochores Appears Soon After Nuclear Envelope Breakdown And Persists Until Late AnaphaseMitotic Location Also Requires Sumoylation |
| Alternative Protein Names | Ran Gtpase-Activating Protein 1 proteinRangap1 proteinRANGAP1 proteinKIAA1835 proteinSD protein |
Information sourced from Uniprot.org