Human Pro-GRP (Pro-GRP) Antibody Pair (STJAP00907)
General Information
| Short Description | This Pro-GRP antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R06-5L-2/R05-8J-4 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Purification |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | GRP |
| Gene ID | 2922 |
| Uniprot ID | GRP_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Function | Stimulates the release of gastrin and other gastrointestinal hormones. Contributes to the perception of prurient stimuli and to the transmission of itch signals in the spinal cord that promote scratching behavior. Contributes primarily to nonhistaminergic itch sensation. In one study, shown to act in the amygdala as part of an inhibitory network which inhibits memory specifically related to learned fear. In another study, shown to act on vasoactive intestinal peptide (VIP)-expressing cells in the auditory cortex, most likely via extrasynaptic diffusion from local and long-range sources, to mediate disinhibition of glutamatergic cells via VIP cell-specific GRPR signaling which leads to enhanced auditory fear memories. Contributes to the regulation of food intake. Inhibits voltage-gated sodium channels but enhances voltage-gated potassium channels in hippocampal neurons. Induces sighing by acting directly on the pre-Botzinger complex, a cluster of several thousand neurons in the ventrolateral medulla responsible for inspiration during respiratory activity. Neuromedin-C: Induces an itch response through activation of receptors present on mast cells, triggering mast cell degranulation. |
| Protein Name | Gastrin-Releasing PeptideGrp Cleaved Into - Neuromedin-CGrp-10Grp18-27 |
| Database Links | Reactome: R-HSA-375276Reactome: R-HSA-381771Reactome: R-HSA-416476 |
| Cellular Localisation | SecretedCytoplasmic VesicleSecretory Vesicle LumenCell ProjectionNeuron ProjectionIn Neurons Of The Retrotrapezoid Nucleus/Parafacial Respiratory GroupExpressed On Neuron Projections Which Project Into The Pre-Botzinger Complex |
| Alternative Antibody Pair Names | Gastrin-Releasing Peptide antibody pairGrp Cleaved Into - Neuromedin-C antibody pairGrp-10 antibody pairGrp18-27 antibody pairGRP antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human Pro-GRP (Pro-GRP) Antibody Pair (STJAP00907)
This antibody pair is designed to detect human Pro-GRP (Pro-Gastrin-Releasing Peptide), corresponding to UniProt entry P07492. Reactivity has been confirmed for human samples only.
Both antibodies are unconjugated rabbit monoclonal IgGs, affinity purified and formulated in 0.01M PBS at pH 7.2. The clone IDs are R06-5L-2 and R05-8J-4.
This pair has been validated for use in ELISA. It has not been validated for other assay formats based on the available product data.
Both antibodies were raised against a recombinant protein immunogen corresponding to human Pro-GRP. No further detail on the specific immunogen region is provided in the current product data.
Working concentrations are not pre-defined for this pair and must be determined empirically by the end user for their specific assay conditions and sample types.
The antibodies should be stored at 4°C for short-term use. For long-term storage, they should be aliquoted and kept at -20°C. Repeated freeze/thaw cycles should be avoided to maintain activity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.